摘要
目的 观察血管内皮生长因子（VEGF）基因、骨髓间充质干细胞（BMSCs）和nano-羟基磷灰石（HA）/聚乳酸羟基乙酸（PLGA）支架材料构建可分泌促进成血管活性细胞因子能力工程骨的相容性.方法 以BMSCs为靶细胞进行VEGF基因转染,观察转染及基因的表达情况.将BMSCs及转染细胞与nano-羟基磷灰石（HA）/聚乳酸羟基乙酸（PLGA）支架材料复合,观察细胞与支架材料的复合情况.结果 转染结果显示空白质粒转染率为39.1％、VEGF组为40.1％.接种5d后,细胞的黏附在支架孔壁上,两组细胞黏附数量差异无统计学意义（P＞0.05）.结论 BMSCs细胞转染VEGF基因后,能够良好存活并与nano-HA/PLGA支架材料复合,高效率表达VEGF,为保证工程骨具有良好生物活性提供必要条件.
Objective To explorec Histocompatibility of nano-hydroxyapatite/poly-co-glycolic acid tissue engineering bone modified by mesenchymal stem cells with vascular endothelial frowth factor transinfected.Methods Rat bone marrow mesenchymal stem cells （BMSCs） was separated,using BMSCs as target cells,and then vascular endothelial growth factor （VEGF） gene was transfected.Composite bone marrow mesenchymal stem cells and cells transfected with nano-hydroxyapatite （HA）/polylactic-co-glycolic acid（PLGA）.The composition of cell and scaffold was observed.Results The blank plasmid transfection was 39.1%,40.1% in VEGF group.The cell adhesion and growth was found on the scaffold pore wall after 5 days,and the number of adherent cells in the nano-HA/PLGA composite scaffold material basically had no significant difference in both.Conclusion Although the nano-HA/PLGA scaffold material is still not fully meet the requirements of the matrix material for bone tissue engineering,but good biocompatibility,structure is its rich microporous satisfaction in material mechanics,toughening,enhanced obviously.Composition scaffold with BMSCs transfected by VEGF plasmid,the ability of angiogenesis is promoted.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2015年第37期3061-3065,共5页
National Medical Journal of China
关键词
骨
人工器官
内皮生长因子
基因
调节
生物医学工程
Bone
Artificial organs
Endothelial groeth factors
Genes,regular
Biomedical engineering
