摘要
植物bHLH(碱性螺旋环螺旋)转录因子是植物体内一类重要的转录因子,在植物的生长发育以及胁迫应答过程中发挥着重要的调控作用。本研究以茶树品种陕茶一号为材料,采用同源克隆法从陕茶一号叶片cDNA中克隆了1个bHLH转录因子CsbHLH2。生物信息学分析表明,CsbHLH2开放阅读框(ORF)为714 bp,编码1个297个氨基酸的蛋白,预测分子量58.4 kD,等电点为5.14,氨基酸序列比对显示该蛋白与其他高等植物的b HLH蛋白具有较高同源性。拟南芥原生质体亚细胞定位表明,CsbHLH2编码蛋白定位在细胞核上。实时荧光定量PCR结果分析表明,CsbHLH2基因在茶树不同组织部位中均有表达,但是在幼叶中表达量最高;不同激素处理结果显示,CsbHLH2受SA、ETH、MEJA诱导。
The bHLH(basic Helix-Loop-Helix) transcription factor is one of the most important transcription factor in plants, which playimportant rolesin plant growth andstressregulation. ThebHLH transcription factorCsbHLH2 wascloned fromtea plant (Camellia sinensis) cultivars‘Shanchayihao’byhomologous cloningtechnologyusing cDNA template. Bioinformatics analysis showed thattheCsbHLH2contains714bpORFandwas predictedencoding 297amino acid,the deduced protein molecular weight was58.4kD and its theoretical isoelectric point was5.14. Amino acid sequence alignment revealed thatCsbHLH2was highlyhomologous to other higher plantbHLH proteins. Transient expression of recombinant plasmidCsbHLH2/PBI221-GFPinArabidopsisprotoplasts showed that CsbHLH2was located in cell nuclei.Quantitative real-time PCR analysis of the expression profiles showed that the CsbHLH2gene wasexpressed in bud, leaf,stem and root.The highest expression level of theCsbHLH2wasfound in the youngleaf.Different hormone treatmentsresults showedthattheCsbHLH2was induced by ETH, MEJA and SA treatment, respectively.
出处
《茶叶科学》
CAS
CSCD
北大核心
2015年第5期481-490,共10页
Journal of Tea Science
基金
国家茶叶产业技术体系(CARS-23)
陕西省科技统筹创新(KTZB02-01)
关键词
茶树
bHLH转录因子
亚细胞定位
进化分析
定量表达分析
Camellia sinensis(L.),bHLH transcription factor,subcelleular locatlization,phylogenetic analysis,expression analysis
