摘要
目的探讨小标本取材方式在非小细胞肺癌表皮生长因子受体（EGFR）基因突变检测价值中的可靠性。方法选取我院2012年6月至2015年5月收集337例进行EGFR基因突变检测的患者资料，统计有效人群资料、吸烟状况、病理类型、EGFR基因突变情况和标本取材方式。结果306例有效样本中，EGFR基因突变率女性45．7％（43／94），男性14．6％（31／212）；从不吸烟人群44．0％（51／116），经常吸烟人群11．8％（20／169）；腺癌35．2％（58／165），鳞癌8．0％（9／112）。突变主要集中在19号外显子的缺失和21号外显子的点突变，性别、吸烟状况、病理类型都是EGFR基因突变的独立影响因素，各组比较差异均有统计学意义，与国内外其他临床研究总体结果相符。结论非小细胞肺癌小标本取材进行EGFR基因突变检测具有较高的可靠性，可作为EGFR基因突变检测的有效取材方式。
Objective To explore the reliability of small specimens in detecting epidermal growth factor receptor （EGFR） gene mutation in non-small cell lung cancer. Methods Data from 337 patients who had accepted the EGFR gene mutation test in Tangdu hospital from June 2012 to May 2015 were collected. Clinical features of those patients had been analyzed concerning gender, smoking status, pathological type,EGFR gene mutation result and specimen source. Results 306 effective samples were selected from all cases and there were 45.7% female patients mutated in EGFR gene while 14.6% in men. The EGFR gene mutation ratios in never smoking and regular smoking smokers were 44.0% and 11.8% ,respectively. Considering the pathological type, there were 35.2% adenocarcinoma and 8.0% squamous carcinoma patients mutated in EGFR gene. Besides, EGFR gene mutations were mainly concentrated as El9 deletion and E21 mutation. In addition,results from the present research also showed that gender, smoking status and pathological type were independent factors for EGFR gene mutation （ P〈 0.05） ,which was consistent with previous clinical researches. Conclusions Small specimens are reliable which can be adopted in detecting EGFR mutation in non-small cell lung cancer patients. [Key words] Non-small cell lung cancer;Epidermal growth factor receptor;Gene mutation
出处
《国际呼吸杂志》
2015年第19期1441-1445,共5页
International Journal of Respiration
关键词
非小细胞肺癌
表皮生长因子受体
基因突变
Non-small cell lung cancer
Epidermal growth factor receptor
Gene mutation
