摘要
为了研究补饲糖蜜尿素舔砖对放牧羊瘤胃中细菌种群结构随时间变化的规律,试验采用基于16S DNA的PCR变性梯度凝胶电泳(DGGE)技术,在放牧羊舔食舔砖的不同时间点取样,进行瘤胃液的DGGE分析。结果表明:舔食糖蜜尿素舔砖后放牧羊瘤胃细菌主要以拟杆菌门、硬壁菌门为主。在舔食15 d后瘤胃细菌多样性显著增加,舔食30 d后瘤胃细菌多样性达到最高。糖蜜-尿素对瘤胃微生物种群具有明显的筛选作用,舔食前以Capnocytophaga canimorsus为优势菌群,舔食一段时间后各菌群或出现或消失,呈动态变化和定植过程;30 d后瘤胃种群结构基本保持稳定,Prevotella ruminicola Bryant处于优势地位。说明舔食舔砖前后细菌种群结构存在明显差异,舔砖可明显增加瘤胃微生物多样性。
To study the time- varying law of bacterial community structures in the rumen of grazing sheep supplemented with molasses -urea lick blocks, a polymerase chain reaction denaturing gradient gel electrophoresis （ PCR - DGGE） technique based on 16S DNA was used for the DGGE analysis of ruminal fluids, which were fetched at different time points when grazing sheep licking molasses - urea. The results showed that the Bacteroidetes and Firmicutes were dominant rumen bacteria in grazing sheep after licking molasses - urea. The diversity of ruminal bac- teria was significantly increased after licking molasses - urea of 15 d, and then the diversity reached the highest value after licking molasses - urea of 30 d. Molasses - urea had a significant role in screening the communities of rumcn microorganisms. Capnocytophaga canimorsus was a dominant bacterial community before grazing sheep licking molasses - urea. Rumen bacteria appeared dynamic changes when grazing sheep lick- ing molasses - urea for a period of time, some appeared and some disappeared. The bacterial community structures of rumen basically remained stable after 30 d, and Prevotella ruminicola （ Bryant et al. ） was in a dominant position. The results indicate that there is an obvious difference in the bacterial community structures between before and after grazing sheep licking the lick block, and the lick block can significantly increase the diversity of rumen microorganisms.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2015年第10期112-115,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
现代肉羊产业技术体系建设专项(CARS-39)
内蒙古自然科学基金项目(2012MS0417)
关键词
放牧羊
糖蜜尿素舔砖
PCR变性梯度凝胶电泳(DGGE)
细菌种群结构
微生物多样性
grazing sheep
molasses - urea lick block
polymerase chain reaction denaturing gradient gel electrophoresis （ PCR - DGGE ）
bac-terial community structure
microbial diversity
