摘要
[目的]用秋水仙素诱导晚抽薹不结球白菜二倍体,以期创制抽薹晚、优质、高产的不结球白菜四倍体新种质。[方法]采用1.5和2.0 g·L-1秋水仙素分别对二倍体不结球白菜茎尖生长点进行4次和6次点滴处理,并鉴定筛选同源四倍体。利用实时荧光定量(qRT-PCR)技术检测抽薹基因的表达,测定营养品质和农艺性状,筛选晚抽薹且优质四倍体材料。[结果]用2.0 g·L-1秋水仙素处理二倍体茎尖6次的效果最好,四倍体的诱导率为5.90%;与二倍体相比,四倍体的叶片、花器、角果和种子都呈现明显差异。流式细胞仪鉴定二倍体(2n=2x=20)DNA相对含量为200,四倍体(2n=4x=40)为400。四倍体抽薹时间比二倍体晚。RT-PCR结果表明:在二、四倍体中Bc FLC3和FRI基因表达随春化时间的延长下调,而VIN3表达上调,四倍体始终高于二倍体;四倍体可溶性糖、可溶性蛋白、维生素C、游离氨基酸和硒含量分别比二倍体显著增加了75.63%、23.84%、44.66%、47.47%和53.79%,有机酸、纤维素和干物质百分比分别降低10.67%、17.56%和12.5%;四倍体单株质量、开展度、叶宽、叶质量、十叶厚、叶柄长和短缩茎质量7个农艺性状与二倍体差异显著,产量增加11.13%。[结论]四倍体不结球白菜表现出营养价值高、农艺性状好且晚抽薹的特性。
[Objectives]Diploid late-bolting plants of non-heading Chinese cabbage were induced by colchicine to creat new germplasm having late-bolting character,high quality and high yield. [Methods]The stem tip growing point of diploid non-heading Chinese cabbage were dripped with 1. 5 and 2. 0 g·L-1colchicine for 4 and 6 times,respectively,then identificated and selected. Different expression levels of bolting genes were checked using RT-PCR. In addition,nutrition was measured and agronomic characters were investigated to create high quality and late-bolting tetraploid. [Results]The result showed that the most effective treatment was2.0 g·L-1for 6 times with induction ratio of 5.90%. Compared with diploid plants,autotetraploid plants had significant differences on leaf,stoma,floral organ and silique. Flow cytometry made clear that the DNA relative content of diploid（ 2n = 2x = 20） was at 200,and tetraploid（ 2n = 4x = 40） was at 400. Tetraploid bolted later than diploid. The result of qRT-PCR indicated that the expression of Bc FLC3 and FRI were decreased but VIN3 was inceased with vernalization,and that tetraploid was higher than diploid all the way. Tetraploids soluble sugar,soluble protein,vitamin C,free amino acids and selenium content significantly increased by 75.63%,23.84%,44.66%,47.47% and 53.79% more than diploid. Organic acids,cellulose and dry matter reduced by 10.67%,17.56% and 12.5%. Tetraploid＇s plant weight,leaf weight,plant width,leaf width,thickness of leaf,petiole length and short stem weight were significantly differrent with diploid,and the yield of tetraploid increased by 11. 13%. [Conclusions]Compared with diploid,tetraploid displayed higher nutritive value,better agronomic traits and late-bolting character.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2015年第5期757-763,共7页
Journal of Nanjing Agricultural University
基金
国家科技支撑计划项目(2012BAD02B01-7)
关键词
不结球白菜
四倍体
晚抽薹
RT-PCR
农艺性状
non-heading Chinese cabbage
tetraploid
late-bolting
RT-PCR
agronomic traits
