摘要
目的观察长链非编码RNASPRY4-IT1（1ncRNASPRY4-IT1）对结肠癌细胞株SW48恶性生物学行为的影响。方法合成针对IncRNASPRY4-IT1的特异性小干扰RNA（si-SPRY4-IT1），转染SW48细胞，利用实时定量反转录聚合酶链反应（RT-qPCR）技术检测SW48细胞中SPRY4-IT1表达量，验证siRNA效果；细胞计数试剂盒（CCK-8）增殖实验检测低表达IncRNASPRY4-IT1对SW48细胞增殖能力的影响；膜联蛋白V-异硫氰酸荧光素（AnnexinV-FITC）／碘化丙锭（PI）染色流式检测法检测对凋亡的影响；Transwell侵袭实验观察对细胞侵袭能力的影响。结果RT-qPCR证实与对照组比较（0．923±0．025），si-SPRY4-IT1可使IncRNASPRY4-IT1表达量（0．3124-0．034）明显降低；CCK-8增殖实验中，IncRNASPRY4-IT1低表达可抑制SW48细胞的增殖能力（P〈0．05）；凋亡实验中，低表达SPRY4-IT1细胞的凋亡率[（15．140±0．680）％]较对照组细胞凋亡率[（4．223±0．724）％]明显增高（P〈0．05）；侵袭实验中低表达SPRY4-IT1细胞穿膜数目为（29．6±4．2）个，较对照组（92．3±2．5）个明显减少（P〈0．05）。结论低表达IncRNASPRY4-IT1可抑制结肠癌细胞SW48增殖和侵袭能力，促进其凋亡。
Objective To investigate the effects of long non- coding RNASPRY4 -IT1 （lncRNA SPRY4 - IT1 ） on malignant biological behaviors of colonic cancer cell line SW48. Methods Synthesized small interfering RNA （siRNA） targeting lncRNA SPRY4 - IT1 （ si - SPRY4 - IT1 ） was transfected into SW48 cells, then the expression of lncRNA SPRY4 - IT1 was detected by real - time quantitative reverse tran- scriptase- polymerase chain reaction （RT- qPCR）. The cell viability was detected by cell counting kit -8 （CCK- 8）, the cell apoptosis was detected by Annexin V -fluoresceine isothiocyanate （FITC）/propidium iodide （PI） and the cell invasion was examined by Transwell assay. Results Compared to the control, the expression of lncRNA SPRY4 - IT1 was significantly decreased in the experimental group （0. 312± 0. 034 vs. 0. 923 ± 0. 025 ）. The proliferation assay showed the proliferation ability of the experimental group was significantly decreased as compared with the control group （P 〈 0. 05 ）. The si - SPRY4 - IT1 promoted cell apoptosis and inhibited migration （P 〈 0. 05 ）. Conclusion Down - regulation of SPRYg - IT1 could in- hibit the proliferation and invasion capability, and promote the apoptosis of human colonic cancer cell SW48.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第10期2450-2452,共3页
Chinese Journal of Experimental Surgery
