摘要
目的:探讨炎症痛模型大鼠外周背根神经节(dorsal root ganglion,DRG)神经元细胞动作电位时空特性的改变。方法:大鼠单侧足底皮下注射福尔马林试剂致痛后,使用膜片钳技术对急性分离的大鼠腰5、腰6背根神经节神经元进行动作电位(action potential,AP)记录。结果:福尔马林组DRG神经元动作电位个数与生理盐水对照组相比明显增加,差异有显著统计学意义(P<0.01);福尔马林组神经元动作电位峰值与生理盐水对照组比较显著降低,差异有显著统计学意义(P<0.01);福尔马林组诱导动作电位爆发的阈电位与生理学盐水对照组阈值相比降低,差异有显著统计学意义(P<0.01);静息电位水平及动作电位时程福尔马林组与生理盐水对照组比较差异无统计学意义(P>0.05)。结论:炎性痛的出现是由于初级感觉神经元兴奋性增加导致外周敏化所致。
Objective：To explore changes in the space and temporal characteristics of action potentials in the dorsal root ganglia （DRG） neurons of rats with inflammatorg pain.Methods：After injection of formalin into the rat hindpaw plantar subcutaneously,the lumbar 5,6 dorsal root ganglion neurons were acutely isolated for patch clamp recordings of action potential （AP）.Results：Number of action potentials of DRG neurons in the formalin model rat was significantly much more than that in the saline treated group （P ＜ 0.01）,while the peak amplitude of the AP in neurons of formalin pain group was less than that of the saline treated group （P ＜ 0.01）.The AP threshold in the formalin group was less than that of the saline treated group （P ＜ 0.01）,but there was no difference in resting potential and duration of AP between formalin pain group and the saline treated group DRG neurons （P ＞ 0.05）.Conclusion：Inflammatory pain is due to the peripheral sensitization caused by the emergence of the increasing of the excitability in primary sensory neurons.
出处
《中国疼痛医学杂志》
CAS
CSCD
2015年第9期658-661,共4页
Chinese Journal of Pain Medicine
