摘要
目的调查金黄色葡萄球菌对夫西地酸的耐药情况。方法收集我院2009年1月到2013年9月分离的金黄色葡萄球菌,共555株,用K-B法测定555株金黄色葡萄球菌对夫西地酸的敏感性,用琼脂稀释法测定夫西地酸对耐夫西地酸金黄色葡萄球菌的最小抑菌浓度（MIC值）,用K-B法测定耐夫西地酸的金黄色葡萄球菌对临床常用的几种抗菌药物的敏感性。结果 555株金黄色葡萄球菌中,262株为耐甲氧西林金黄色葡萄球菌（MRSA）,293株为甲氧西林敏感金黄色葡萄球菌（MSSA）。77株金黄色葡萄球菌对夫西地酸耐药,包括60株MRSA和17株MSSA。金黄色葡萄球菌、MRSA和MSSA对夫西地酸的耐药率分别为13.87%（77/555）,22.90%（60/262）和5.80%（17/293）。2009年到2013年金黄色葡萄球菌对夫西地酸的耐药率分别为7.81%（5/64）,9.80%（5/51）,22.92%（11/48）,5.21%（11/211）和24.86%（45/181）。夫西地酸对耐夫西地酸金黄色葡萄球菌的MIC值范围为2-〉64μg·m L-1,其中MIC值〉64μg·m L-1的菌株有44株,占57.14%（44/77）。所有耐夫西地酸的金黄色葡萄球菌对奎诺普丁/达福普汀、万古霉素、利奈唑胺和呋喃妥因均表现为敏感;但对青霉素、四环素、克林霉素、红霉素和环丙沙星等抗菌药物则表现出较高的耐药率（〉50%）。结论夫西地酸仍对金黄色葡萄球菌表现出较好的体外抗菌活性,但其耐药率有增加的趋势,且呈高水平耐药。
Objective To investigate the prevalence of fusidic acid（ FA） resistance among Staphylococcus aureus clinical isolates. Methods Five hundred fifty- five Staphylococcus aureus isolates were isolated from the various specimens of patients at the first affiliated hospital of Wenzhou medical university from January,2009 to September,2013.The susceptibility of Staphylococcus aureus isolates to FA and clinically often used antimicrobials was determined by disc diffusion method. The minimal inhibitory concentrations（ MICs） of FA and vancomycin for FA- resistant isolates was determined by agar dilution method.Results Among 555 Staphylococcus aureus isolates,293 were methicillin- resistant Staphylococcus aureus（ MRSA） and 262 were methicillin- susceptible Staphylococcus aureus（ MSSA）. Seventy- seven of 555（ 13. 87%） isolates including 60 MRSA and 17 MSSA isolates were resistant to FA. The rates of FA resistance among MRSA and MSSA isolates were 22. 90%（ 60 /262） and 5. 80%（ 17 /293）. The rates ofFA resistance among Staphylococcus aureus isolates from 2009 to 2013 were 7. 81%（ 5 /64）,9. 80%（ 5 /51）,22. 92%（ 11 /48）,5. 21%（ 11 /211） and 24. 86%（ 45 /181）,respectively. The MICs of FA for 77 FA- resistant isolates ranged from 2 to 〉64 μg·m L^-1,among which 44 with fusidic acid MIC of 64 μg·m L^-1. All FA- resistant isolates were susceptible to quinupristin- dalfopristin,vancomycin,linezolid and nitrofurantoin. While more than50% of theses isolates were resistant to penicillin, tetracycline, clindamycin, erythromycin and ciprofloxacin.Conclusion FA has fine antimicrobial activity in vitro against Staphylococcus aureus isolates,but the FA resistance among these isolates was increasing,especially increased high- level FA resistance.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第19期1971-1973,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81271906H2002)
关键词
金黄色葡萄球菌
夫西地酸
耐药率
最小抑菌浓度
Staphylococcus aureus
fusidic acid
resistance
minimal inhibitory concentration
