摘要
为深入研究PPARD在猪体内的调节和功能,以版纳微型猪近交系(BMI)为试验动物,通过RT-PCR方法扩增得到PPARD基因全长编码区序列,在编码区第95位检测到A/G两种核苷酸(GenBank登录号:KP757025、KP757026)。结果表明,该基因编码462个氨基酸,蛋白分子量为49.74kD,等电点为7.53。蛋白质结构分析PPARD存在2个保守域,无跨膜结构,不存在信号肽,存在1个亮氨酸富集的核输出信号,其N端、C端均亲水;亚细胞定位显示该蛋白分泌到细胞周质的概率为69.6%。系统进化分析表明猪与牛、犬的亲缘关系较近。利用qPCR检测了PPARD基因mRNA在BMI 14个组织中的表达量,发现在肝脏、耳朵、脾脏、小肠中表达量较高。
PPARD gene plays an important role in animal metabolism. To study the regulation and function of PPARD in pigs, PPARD coding sequence was obtained by RT-PCR and submitted to GenBank with the access number KP757025 and KP757026. Two different nucleotides （A or G） at 95 nucleotide were obtained. Bioinformatics analysis showed that the PPARD gene encode a protein of 462 amino acids with a predicted molecular weight of 49.74 kD and the isoelectrie point of 7.53. Protein structure analysis showed that PPARD protein contained two conserved domains and a leucine-rich nuclear export signal without transmembrane regions and signal sequence. Hydrophobicity analysis indicated that the N-termi- nus and C-terminus were hydrophilic. Subcellular localization analysis concluded that the the probability of secreting into periplasmic was 69.6%. Phylogenetic results discovered that the pigs had the closest rela- tionship with cattle and dogs. PPARD mRNA expression in 14 tissues was tested by qPCR and the results showed that the expression level was high in liver, ear, spleen and small intestine. This study provided experimental basis for further study on the expression and regulation of PPARD gene.
出处
《家畜生态学报》
北大核心
2015年第9期13-19,共7页
Journal of Domestic Animal Ecology
基金
国家自然科学基金项目(31160439
31460580)
