摘要
目的：研究染色体9p21上两个单核苷酸多态性（SNP）位点（rs2383206．rs10757278）在新疆维汉两族人群中的分布，探讨与所有病例中大血管病变（冠状动脉病变、颈动脉硬化及外周动脉病变）发生率的相关性及危险因素；进一步研究在2型糖尿病（T2DM）中维汉两族染色体9p21上两个 SNP 位点（rs2383206． rs10757278）与大血管病变发生率的相关性及大血管病变的相关危险因素。方法选择内分泌科住院 T2DM患者497例，其中维吾尔族298例，汉族199例；心内科非 T2DM 患者215例，其中维吾尔族93例，汉族122例，共712例。用 PCR-SNPStream 技术对 rs2383206．rs10757278位点进行 SNP 分型及分析，对结果进行统计学分析。结果所有大血管病变组中按维、汉分组，两个 SNP 位点的基因型分布差异无统计学意义（rs2383206：χ^2＝5．570，P ＝0．062；rs10757278：χ^2＝2．721，P ＝0．257），所有 T2DM患者中按有无大血管病变分组，两个 SNP 位点的基因型分布差异无统计学意义（rs2383206：χ^2＝0．120，P ＝0．950；rs10757278：χ^2＝1.027，P ＝0．598）。Logisitic 逐步回归分析显示，维族 T2DM患者大血管病变与增龄相关（χ^2＝28．820，P ＝0.000），与脂肪肝相关（χ^2＝5．210，P ＝0．020）；汉族 T2DM患者大血管病变与增龄（χ^2＝19．980，P ＝0．000）、空腹血糖（FPG）水平较高（χ^2＝4．070，P ＝0．044）、糖化血红蛋白控制不佳相关（χ^2＝4．280，P ＝0．040）。结论染色体9p21上两个 SNP 位点（rs2383206．rs10757278）与维、汉所有病例大血管病变无相关性。增龄、FPG 升高、糖化血红蛋白控制不佳伴脂肪肝是大血管病变发生的危险因素。
Objective To assessed the distribution of two single nucleotide polymorphism （SNP）loci （rs2383206.rs10757278）on chromosome 9p21 in Xinjiang Uygur and Han nationality populations,and to investigate correlation and the incidence of all cases of macrovascular disease （coronary artery disease,carotid atherosclerosis and peripheral arterial disease）and analysis of risk factors.To further study the correlation between the incidence of two single nucleotide polymorphism （SNP）loci （rs2383206.rs10757278）on chromosome 9p21 in type 2 diabetes melli-tus（T2DM）of Han and Uygur ethnic and the incidence of all cases vascular disease,then to analysis the risk factors. Methods 497 adults with T2DM who were treated in the Endocrinology department in hospital from May 2012 to April 2014 were involved in this study,including 298 Uygur patients and 199 Han patients.215 non -T2DMpatients who were treated in the Cardiology department in hospital were also involved in the study,including 93 Uighur patients and 122 Han patients.Then the total 712 patients were detectedby using PCR -SNP Stream technology to analyse rs2383206.rs10757278 loci SNP genotyping.The relevant results were compared with t test,two different genotype distribution and allele frequency were compared with χ^2 test,multiple factors analysis were calculated by Logisitic regression.Results The distribution of genotype with two SNP loci had no significant difference between the patients in Uygur group and Han group （rs2383206χ2 =5.570,P =0.062;rs10757278 χ^2 =2.721,P =0.257 ）,and there＇s no significant difference between the patients with macrovascular disease and non -macrovascular disease in all patients（rs2383206χ^2 =0.120,P =0.950;rs10757278 χ^2 =1.027,P =0.598）.Logisitic regression analysis showed that the incidence of macrovascular was significantly associated with increasing age（χ^2 =28.820,P =0.000）and fatty liver（χ^2 =5.210,P =0.020）in Uighur group with type 2 DM.In Han group with type 2 DM,the macrovascular was sig
出处
《中国基层医药》
CAS
2015年第18期2733-2737,共5页
Chinese Journal of Primary Medicine and Pharmacy
基金
基金项目：新疆维吾尔自治区乌鲁木齐市人口与健康科技专项基金项目（Y111310027）
