摘要
目的:探讨盐水介质法、凝聚胺法及微柱凝胶法在新生儿输血安全中的应用价值。方法:选取2014年2月至2015年5月我院新生儿血液标本1 732份,其中弱抗体血浆标本87份,用3种方法进行交叉配血,同时检测弱抗体血浆标本与相应标准RBC反应效果。配血不合则做直接抗人球蛋白试验和抗体筛选、鉴定试验。结果:盐水介质法、凝聚胺法及微柱凝胶法3种方法共出现82例配血不合的阳性结果,分别检出为14例、34例和59例;漏检不规则抗体分别为24例、4例和2例;真正配血不合不能输血56例;凝聚胺法弱抗体检出率为52.87%,明显低于盐水介质法和微柱凝胶法的81.61%和89.66%,差异比较有统计学意义(P<0.05);后两种方法检出率差异比较无统计学意义(P>0.05);3种方法检测的灵敏度和特异性为14.29%和99.64%,50.00%和99.64%,96.43%和99.70%。结论:3种交叉配血法均有各自的优缺点,其中微柱凝胶法敏感性高,操作简便快速,安全有效。
Objective： To investigate the application value of saline medium,coagulation amine and raicro-gel method in neonatal transfusion safety.Methods：From February 2015 to May 2014 in our hospital, selectd blood samples of 1732 newborns,wherein the weak antibody plasma samples 87,used three methods crossmatch, simultaneously detected weak antibody plasma samples with the appropriate standards RBC response effect.Bloodincompatibility then maked direct antiglobutin test,antibody screening and identification test.Results：And the detection rate was 81.61% of 56 patients were unsuited to the saline medium method, polybrene method and micro column gel method 3 kinds of methods there were 82 cases with blood positive results were detected in 14 cases,34 cases and 59 cases; omission of irregular antibodies in 24 cases, 4 cases and 2 cases,true with incompatible blood could not be a blood transfusion;polybrene method weak antibody positive rate was 52.87%,significantly lower than saline medium method and micro column gel method and 89.66%,the difference was statistically significant （P＇〈0.05）; the latter two methods the difference was not statistically significant （P〉0.05）; 3 kinds of methods for detection of sensitivity and specificity was t4.29% and 99.64%, 50.00% and 99.64% and 96.43% 99.70%.Conclusion：Three kinds of cross matching method has its own advantages and disadvantages,micro-gel method with high sensitivity and simple,rapid,safe and effective.
出处
《中国医药导刊》
2015年第9期895-896,共2页
Chinese Journal of Medicinal Guide
