摘要
目的:探讨Gab2过表达对人结直肠癌细胞株SW480增殖和迁移能力的影响。方法:用携带Gab2基因的重组慢病毒颗粒(LV-Gab2-GFP)感染人结直肠癌SW480细胞株,作为实验组(LV-Gab2-GFP组);以对照慢病毒颗粒(LV-GFP)感染人结直肠癌SW480细胞株,作为阴性对照组(LV-GFP组);空白对照组常规培养,不作任何处理。用RT-PCR和Western blot法分别检测细胞中Gab2 mRNA和蛋白表达的水平;CCK-8法和平板克隆实验检测细胞增殖能力的变化;划痕愈合实验检测细胞迁移能力的变化。结果:RT-PCR和Western blot均显示LV-Gab2-GFP组Gab2的表达均较对照组显著增高;与对照组比较,Gab2过表达显著增强人结直肠癌SW480细胞的增殖和迁移能力。结论:Gab2过表达可以促进SW480细胞增殖和迁移能力。
Objective：To investigate the effects of Gab2 overexpression on the proliferation and migration of human colorectal cancer cell line SW480.Methods： The experimental group （LV-Gab2-GFP group）,colorectal cancer SW480 cells were transfected with recombinant lentivirus vector （LV-Gab2-GFP）,the negative control group was transfected with negative control lentiviral vector （ LV-GFP） ,and the blank control group without any treatment.The mRNA and protein expression of Gab 2 in cells were identified by RT-PCR and Western blot respectively.Proliferation of the cells was detected by CCK-8 colorimeter and colony forming assay.Wound-healing assay was used to determine the cells migration .Results： RT-PCR and Western blot demonstrated that Gab 2 mRNA and protein expression significantly increased in LV-Gab2-GFP group compared with control groups;overexpression of Gab2 markedly enhanced human colorectal cancer SW 480 cells proliferation and migration compared with control groups .Conclusion：Overexpression of Gab2 accelerates human colorectal cancer SW 480 cells proliferation and migration.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第9期1210-1213,共4页
Chinese Journal of Immunology
基金
贵州省科学技术基金项目(黔科合J字[2013]2312)资助
关键词
Gab2
结直肠癌
慢病毒
增殖
迁移
Gab2
Colorectal cancer
Lentivirus
Proliferation
Migration
