摘要
目的：建立鹅不食草中有效成分短叶老鹤草的定量分析方法。方法：采用反相高效液相色谱法测定含量,色谱柱为Kromasil C18色谱柱（250mm×4.6mm,5μm）,流动相为乙腈-水（45∶55）,流速：1.0m L/min;柱温：30℃;进样量：10μL;检测波长：225nm。结果：短叶老鹤草在0.010065～0.30195mg/m L范围内线性关系良好（r=1.000）,平均回收率（n=6）为99.53%,样品含量范围为0.048%～0.43%。结论：建立的方法简单灵敏,准确可靠,重复性好,可用于鹅不食草的质量控制。
objective：To establish quantitative analysis of brevifolin in CENTIPEDAE HERBA. Methods：Quantitative analysis was performed on Kromasil C18 （250mm×4.6mm,5μm）.The mobile phase was aeetonitrile-water （45：55）at the flow rate of 1.0mL·min^-1, the detection wavelength was225nm, the column temperature was 30℃.Results： Brevifolin showed good linear regression （r=1.000）within test ranges of 0.010065-0.30195mg/mL.The average recovery （n=6）was 99.53% ,and the range of contents in the samples were 0.048%- 0.43% . Conclusion：The methods developed are sensitive ,reliable and useful for the analysis of samples purchased from different areas and can be used for the quality control of CENTIPEDAE HERBA.
出处
《北方药学》
2015年第10期8-9,共2页
Journal of North Pharmacy
