期刊文献+

乳源嗜冷菌产胞外耐热酶检测方法的对比分析 被引量:3

Comparing Detection Methods for Extracellular Thermostable Enzymes Secreted by Psychrophile in Milk
下载PDF
导出
摘要 乳及乳制品中污染的嗜冷菌可分泌耐热的胞外蛋白酶和脂肪酶,直接影响产品品质。介绍从乳体系中分离鉴定的嗜冷菌种类,指出荧光假单胞菌是产胞外蛋白酶和脂肪酶的主要嗜冷菌菌株;分别阐述乳中污染的嗜冷菌所分泌蛋白酶和脂肪酶的热稳定性,并比较乳体系中耐热酶的测定方法,期望为有效预测乳中嗜冷菌污染程度、在线检测和控制耐热酶活性、提升乳制品的质量提供理论参考。 Extracellular protease and lipase could be secreted by psychrotrophic bacteria in dairy products. These enzymes directly influenced the quality of dairy products. The study described the species of psychrophile isolated from milk, pointing out that pseudomonas fluorescens were the main strains which could metabolize protease and lipase. Thermal stability of these enzymes was elaborated and the methods of detection them were compared. It was expected to provide theoretical thoughts to predict the amount of contaminated psychrophile in dairy and dairy products, online detect and control the activity of thermostable enzyme and improve the quality of dairy products.
出处 《食品研究与开发》 CAS 北大核心 2015年第15期87-91,共5页 Food Research and Development
关键词 嗜冷菌 蛋白酶 脂肪酶 检测方法 milk psychrophile protease lipase detection
  • 相关文献

参考文献49

  • 1张瑞宇,刘立衡.灭菌牛乳中脂肪酶活性变化的研究[J].食品科学,2009,30(17):221-224. 被引量:9
  • 2Chena L, Daniela RM, Coolbearb T. Detection and impact of pro- tease and lipase activities in milk and milk powders[J]. Int Dairy J, 2003, 13:255-275. 被引量:1
  • 3Ercolini D, Russo F, Ferrocino I, et al. Molecular identification of mesophilic and psychrotrophie bacteria from raw cow's milk [J]. Food Microbiol, 2009, 26:228-231. 被引量:1
  • 4Elionora H Z, Malka I4. Culturable psychrotrophic bacterial commu- nities in raw milk and their proteolytic and lipolytic traits[J]. Appl Environ Microbiol, 2007, 73(22): 7162-7168. 被引量:1
  • 5Kumaresan G, Annalvilli R, Sivakumar K, et al. Psychrotrophic spoilage of raw milk at different temperatures of storage[J]. J Appl Sei Res, 2007, 3(11): 1383-1387. 被引量:1
  • 6Martin W, Denise W. Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk [J]. Appl Environ Microbiol, 2000, 66(5): 2085-2095. 被引量:1
  • 7Swart G J,Jooste P J,Mostert J F,et al. Species identification and some physiological characteristics of Gram-negative psychrotrophic isolates from raw silo milk[J]. Suid-Afrikaanse Tydskrif vir Sui- welkunde, 1990, 22:31-36. 被引量:1
  • 8Fairbairn D J, Law B A. Proteinases of psychrotrophic bacteria: Their production, properties, effects and control[J]. J Dairy Res, 1986, 53 (1): 139-177. 被引量:1
  • 9Tondo E C, Lakus F R, Oliveira F A, et al. Identification of heat sta- ble protease of Klebsiella oxytoca isolated from raw milk [J]. Lett Appl Microbiol, 2004, 38(2): 146-150. 被引量:1
  • 10Griffiths M W,Phillips J D,Muir D D. Thermostability of proteases and lipases from a number of species of psychrotrophic bacteria of dairy origin[J]. J Appl Baeteriol, 1981, 50(2): 289-303. 被引量:1

二级参考文献19

共引文献17

同被引文献32

引证文献3

二级引证文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部