摘要
目的对比研究机械灌注与单纯冷保存对猪心脏死亡器官捐献(DCD)供胰的影响。方法健康猪10只,随机分为单纯冷保存组和机械灌注组两组(每组各5只)。制备DCD猪模型,胰腺切取后采用威斯康星大学保存液(UW液)保存。单纯冷保存组给予单纯UW液冷保存,机械灌注组给予机械灌注保存。分别于保存1、2、3、4、6、24 h时点在胰尾部取材,制作组织切片,予苏木素-伊红(HE)染色。两组进行胰腺组织病理学检查,并对病理学评分进行比较。结果猪DCD供胰在机械灌注180 min时胰腺微血栓已被清除,又避免了过度灌注对胰岛的损害。机械灌注组病理学评分为(4.2±0.8)分,单纯冷保存组病理学评分为(8.4±1.1)分,比较差异有统计学意义(P<0.05)。结论机械灌注可以有效清除胰腺血管内血栓。与单纯冷保存比较,保存相同时间后,机械灌注组更能维持胰岛的完整性。
Objective To compare the effect of mechanical perfusion and simple cold preservation on donation after cardiac death （DCD）pancreas in pigs.Methods Ten healthy pigs were randomized into simple cold preservation group and the mechanical perfusion group （5 pigs in each group ）.DCD model was established in pigs.The pancreas was cut and stored in University of Wisconsin solution （UW solution）.The pancreas of the simple cold preservation group was preserved with simple UW solution and that of the mechanical perfusion group was preserved by mechanical perfusion.Specimen was collected from pancreatic tail at 1,2,3,4,6 and 24 h to prepare tissue section.Then,the tissue section was stained with hematoxylin-eosin （HE）.Histopathological examination was conducted and pathological score of the two groups was compared.Results Microthrombus in DCD pancreas of pig was removed at 180 min of mechanical perfusion and injury to pancreas islet caused by excessive perfusion was avoided.The pathological score of the mechanical perfusion group was （4.2 ±0.8）and that of the simple cold preservation group was （8.4 ±1.1）,and the difference had statistical significance （P 〈0.05）.Conclusions Mechanical perfusion may effectively remove thrombus in pancreatic vessels.Compared with simple cold preservation,mechanical perfusion may maintain the integrity of pancreas islet better after the preservation of the same period of time.
出处
《器官移植》
CAS
CSCD
2015年第5期307-310,315,共5页
Organ Transplantation
基金
公益性行业科研专项(201302009)
关键词
胰腺移植
低温
机械灌注
心脏死亡器官捐献
威斯康星大学保存液
Pancreas transplantation
Low temperature
Mechanic perfusion
Donation after cardiac death
University of Wisconsin solution
