摘要
目的:探讨白藜芦醇(resveratrol,RES)对卵巢切除小鼠血脂四项总胆固醇(CHOL)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)以及血清一氧化氮(nitric oxide,NO)含量、胸主动脉的过氧亚硝基阴离子(peroxynitrite anion,ONOO-)及诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)蛋白表达水平的影响。方法:雌性C57小鼠经卵巢切除建立去势模型后,分为假手术组、单纯去势组、假手术高脂组、模型高脂组和RES组。单纯去势组和假手术组给予普通饲料,其余给予高脂饲料。14周后,收集血清检测各组血清的CHOL、TG、LDL-C和HDL-C水平,硝酸还原酶法测血浆NO水平,油红O染色观察动脉粥样硬化(atherosclerosis,AS)情况,DAB染色测定血管ONOO-及iNOS水平,免疫印迹测定血管组织的iNOS表达。结果:模型高脂组血清CHOL、TG、LDL-C和NO较正常对照组升高(P<0.05);与模型高脂组相比,RES组可降低小鼠血清的TC、TG、LDL-C和NO(P<0.05);14周后,AS模型成功建立,模型高脂组有明显的AS病理改变,单纯去势组无明显AS斑块,RES组AS病变明显减轻;高脂喂养14周后,模型高脂组血管组织的iNOS及ONOO–表达也较正常对照组明显提高(P<0.05);与模型高脂组相比,RES组可降低小鼠胸主动脉的iNOS表达(P<0.05),与此同时,血管ONOO-含量较模型高脂组降低。结论:RES可以抑制iNOS表达,减少NO生成,防治动脉粥样硬化。
AIM：To investigate the effect of resveratrol on the lipids （ CHOL, TG, LDL-C and HDL-C） , ni-tric oxide （ NO） , peroxynitrite anion （ ONOO-） and the expression of inducible nitric oxide synthase （ iNOS） in the artery of the mice with ovariotomy （ OVX） .METHODS：The lipid levels and NO level in the serum were measured .The chan-ges of atherosclerosis were evaluated with Oil Red O staining .The expression of iNOS was measured by DAB staining and Western blot .The ONOO-production was measured by DAB staining .RESULTS：Compared with sham group , the levels of the lipids and NO production in OVX ＋high fat （HF） group were increased （P〈0.05）.Compared with OVX＋HF group, the levels of the lipids and NO production in resveratrol group were decreased （P〈0.05）.Fourteen weeks later, the atherosclerosis model was successfully established .Compared with OVX ＋HF group, the iNOS expression and the ONOO-production in resveratrol group were decreased （ P〈0.05 ） , while those in sham group were increased （ P 〈0.05）.CONCLUSION：Resveratrol prevents and treats atherosclerosis by inhibiting the iNOS expression in C 57BL/6J mice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2015年第9期1601-1605,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81202186)
