摘要
目的研究口腔癌相关成纤维细胞(CAFs)在口腔鳞状细胞癌淋巴管生成过程中的作用。方法通过组织块法分离、培养口腔鳞状细胞癌患者的CAFs和正常成纤维细胞(NFs),免疫组织化学染色鉴定CAFs和NFs。利用24孔的transwell细胞培养室分别将CAFs(实验A组)、NFs(实验B组)与人淋巴管内皮细胞(HLEC)共培养,以DMEM高糖培养基作为空白对照组,在倒置相差显微镜下观察各组HLEC的增殖、迁移、侵袭、成管能力。结果培养96、144、192 h,实验A组HLEC的数目均多于实验B组和空白对照组,实验B组多于空白对照组(P<0.01)。培养96 h后,实验A组HLEC的迁移和侵袭数目均多于实验B组和空白对照组,实验B组多于空白对照组(P<0.01)。培养24 h后,实验A组HLEC的成管数目多于实验B组和空白对照组,实验B组多于空白对照组(P<0.01)。结论口腔CAFs促进了HLEC的增殖、迁移、侵袭和成管作用,并且CAFs的促进作用大于NFs。
Objective To investigate the effects of oral cancer-associated fibroblasts （CAFs） on lymphangiogenesis in oral squamous cell carcinoma （OSCC）. Methods CAFs and normal fibroblasts （NFs） were obtained from the tissues of patients with OSCC who did not receive radio-chemotherapy before operation. And the CAFs and NFs were isolated by method of tissue block and identified by immunohistochemical staining. The effects of CAFs （group A） and NFs （group B） to human lymphatic endothelial cells （HLEC） were detected by using a 24-multiwell transwell cell culture chamber. DMEM sugar medium was as blank control group. The number of proliferative, migratory, invasive and tubes of HLEC were counted under inverted phase contrast microscope. Results The proliferative number of HLEC of group A for 96, 144, 196 h was significantly higher than that of group B and blank control group, group B higher than blank control group （P〈0.01）. The migratory and invasive number of HLEC of group A for 96 h was significantly higher than that of group B and blank control group, group B higher than blank control group （P〈0.01）. The number of tube formation of HLEC of group A for 24 h was significantly higher than that of group B and blank control group, group B higher than blank control group （P〈0.01）. Conclusion CAFs promote HLEC＇s proliferation, migration, invasion, tube formation, and these effects are stronger than NFs.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2015年第5期524-528,共5页
West China Journal of Stomatology
