摘要
目的探讨芦丁(Rutin)对脂多糖(Lipopolysaccharide,LPS)诱导的小鼠急性肺损伤(ALI)氧化应激失衡的作用。方法将30只雄性C57小鼠随机分为3组:对照组(C组)、脂多糖组(LPS组)和芦丁组(R组),每组10只。R组在LPS注射前0.5 h给予芦丁(100μmol/kg)腹腔注射,C组和LPS组在LPS注射前0.5 h给予等量生理盐水腹腔注射,LPS组和R组给予脂多糖(20 mg/kg)腹腔注射,C组给予等浓度生理盐水腹腔注射。脂多糖或生理盐水注射后6 h测小鼠血气,取肺组织进行病理观察,测定肺组织湿干比(W/D)、丙二醛(MDA)含量,检测过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)活性,血清和肺组织中肿瘤坏死因子-α(TNF-α)、白介素(IL)-6和IL-1β的表达。结果与C组比较,LPS组肺组织病理改变和肺组织湿干比、二氧化碳分压(Pa CO2)、呼吸频率(RR)、MDA含量及IL-6、IL-1β和TNF-α表达明显增加,而氧分压(Pa O2)、p H值及CAT、GPx、SOD活性明显降低。与LPS组比较,R组肺组织病理改变和肺组织湿干比、Pa CO2、RR、M DA含量及IL-6、IL-1β、TNF-α表达明显减少,而Pa O2、p H值及CAT、GPx、SOD活性明显增高。结论芦丁可通过抑制氧化应激和炎症而减轻脂多糖诱导的急性肺损伤。
Objective To investigate the effects of rutin on oxidative stress of acute lung injury induced by li-popolysaccharide （LPS）. Methods 30 male C57 mice were randomly divided into control group （group C）,group LPS,and rutin group （group R）. Group R was pretreated with rutin （100 μmol/kg）by intraperitoneal injection at 0.5 h before LPS injection,and the equal volume of saline was administered in the other 2 groups. Group R and group LPS were treated with LPS （20 mg/kg）by intraperitoneal injection and group C were treated with the saline （20 mg/kg）by intraperitoneal injection. After 6 h of LPS injection,arterial blood was collected for blood gas analysis and lung tissue to exmanine pathological change,Wet/Dry,the expression of MDA,the activity of CAT,GPx and SOD and the expression of IL-6,IL-1β and TNF-α in lung and serum. Results Compared with group C,the pathological change and Wet/Dry of lung tissue,the PaCO2 and RR,the expression of MDA in lung tissue and the expression of IL-6,IL-1β and TNF-αsignificantly increased,while the PaCO2 ,pH and the activity of CAT,GPx and SOD decreased. Compared with group LPS,the pathological change and Wet/Dry of lung tissue,the PaCO2 and RR,the expression of MDA in lung tissue and the expression of IL-6,IL-1β and TNF-α in group R significantly decreased and the PaCO2,pH and the activity of CAT,GPx and SOD increased. Conclusion Rutin pretreatment can significantly decrease LPS-induced acute lung inju-ry by suppressing oxidative stress and inflammation.
出处
《实用药物与临床》
CAS
2015年第9期1011-1014,共4页
Practical Pharmacy and Clinical Remedies
关键词
芦丁
脂多糖
急性肺损伤
氧化应激
炎症
Rutin
LPS
Acute lung injury
Oxidative stress
Inflammation
