摘要
通过分析大肠杆菌dnaJ缺失菌株在43℃下的生长表型,发现AtDjA5及其J-Domain能够功能性地替换DnaJ及其J-Domain.免疫共沉淀实验结果表明AtDjA5与DnaK存在相互作用.由Western blot检测推测AtDjA5能够稳定大肠杆菌热激转录因子σ32并下调热激蛋白DnaK表达量.这些发现说明AtDjA5在大肠杆菌中具有与DnaJ相似的功能.
By analyzing growth phenotype of the dnaJ mutant strain at 43℃, AtDjA5 and its J-Domain can functionally substitute for DnaJ and its J-Domain. Co-immunopreeipitation experiments show that AtDjA5 can interact with DnaK. It＇ s speculated that AtDjA5 can stabilize the heat shock factor 3z of E. coli and down-regulate the expression of Dnak by western blot analysis. These findings suggest that AtDjA5 has a similar function with DnaJ in E. coli.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第5期1117-1122,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家863项目(2012AA022204)
国家自然科学基金(31171586)
