摘要
目的观察高压氧对动物激素性股骨头坏死的预防作用。方法将35只雄性Wistar大鼠随机分为空白组5只,造模组15只,预防组15只。造模组给予地塞米松磷酸钠注射液腹腔注射10 m L/kg,2次/周,制备激素性股骨头坏死模型;对照组在造模处理基础上同时给予高压氧,压力为1.8 ATA(绝对大气压),每日1次,每次1 h;空白组予以等量生理盐水注射。实验第8周,对所有大鼠行双髋正位X线摄片检查,随后处死动物并取大鼠双侧股骨头,分别行大体和光镜观察。结果 3组大鼠股骨头双髋正位X线摄片及股骨头标本大体外观均未发现异常,在病理切片上可发现显著差异。切片HE染色后光镜观察显示空白组无骨坏死灶,造模组出现明显的骨坏死灶;与造模组比较,预防组骨细胞受损程度较轻。结论高压氧对激素性股骨头坏死的早期骨细胞坏死有一定的防护作用。
Objective To investigate the effects of hyperbaric oxygenation （HBO） on preventing the steroid-induced avascular necrosis of the femoral head （SANFH） of mammals. Methods Distributing the 35 male Wistar rats into 3 groups： 5 rats for blank group, 15 rats for model group, and 15 rats for control group. The rats in the model group and the control group were injected the dexamethasone sodium phosphate injection （10 mL/kg, 2 times/week） in the abdominal cavity to built the model of SANFH. While establishing the rats model, the rats in the control group were given the HBO （pressure： 1.8ATA, 1 time/day, 1 hour/time） at the same time. And the blank group were injected normal saline. After 8 weeks, anteroposterior bilateral hip X- Ray examinations were performed in all of the rats. Then, executed them, took the femoral head out and the general observation and histological study were performed. Results There was no obvious abnormality in the results of the general observation and the X- Ray examinations. But there were visible differences between model group and control group in the pathological sections. After stained with HE, the sections were observed under the light microscope： there were no focal osteonecrosis in the sections of the blank group and obvious focal osteonecrosis in the model group. And comparing with the model group, the osteocytes in the control group had a lighter damage. Conclusion HBO can prevent the damage of osteocytes during earlier period of SANFH.
出处
《中国药业》
CAS
2015年第19期25-27,共3页
China Pharmaceuticals
基金
北京市自然基金资助项目
项目编号:7154241
全军医学科技青年培育项目
项目编号:13qnp075
关键词
高压氧
大鼠
股骨头坏死
激素
hyperbaric oxygenation
rat
femoral head necrosis
glucocorticoid
