摘要
研究了福建地区3种主要蜂蜜(龙眼蜜、荔枝蜜、八叶五加蜜)荧光的稳定性,包括金属离子稳定性、热稳定性以及光稳定性。金属离子稳定性考察了8种外加金属离子(Na+、K+、Ca2+、Mg2+、Cu2+、Zn2+、Pb2+、Cd2+)对蜂蜜荧光的影响,结果显示在考察的浓度范围内(200 mg/kg)8种外加金属离子对蜂蜜的荧光强度均没有显著影响。热稳定性考察了三种温度条件下(50℃、70℃、90℃)蜂蜜荧光随加热时间的变化,结果表明在1h的加热时间内50℃和70℃的加热温度对蜂蜜荧光没有显著影响;在90℃加热条件下,蜂蜜荧光出现增强现象,其增强幅度分别为龙眼蜜45%,荔枝蜜21%,八叶五加蜜1 0%。光稳定实验研究了持续紫外光照对蜂蜜荧光的影响,结果显示三种蜂蜜荧光随光照时间延长均出现不同程度光漂白。其中龙眼蜜和荔枝蜜光照30 min后荧光强度分别下降30%和25%,但光谱形状没有显著变化;八叶五加蜜则发现363 nm处荧光发射峰出现光降解现象,其荧光强度下降了54%,且荧光光谱中出现发射波长位于397 nm的新荧光发射峰。该研究表明蜂蜜荧光具有较好的金属离子稳定性和热稳定性,正常蜂蜜生产和加工过程中可能的金属离子或高温接触不会对其荧光产生影响;此外,在应用荧光光谱技术进行蜂蜜相关研究中样品需要避光保存,以避免长期光照对蜂蜜荧光产生漂白或降解。
The object of this paper is to estimate the stability of the fluorescence from three types of honey harvested in Fujian province （Iongan honey, lichi honey, and schefflera honey）. These stability studies include effects of metal ions, heat treatment, and photo irradiation on fluorescence of honey. Results indicate that extra metal ions （Na＋, K＋, Ca2＋, Mg2＋, Cu2＋. Zn2＋. Pb2＋. Cd2＋） have no influence on the fluorescence of honey as the added concentration is 200 mg/kg. After been heated for I h under 50℃ and 70℃, fluorescence of honey do not show significantly change. However, enhancement of fluorescence intensity is observed under 90℃, and the increase percent is calculated to be 45%, 21%, and 10% for longan honey, lichi honey, and schefflera honey, respectively. In photo-stability experiments. photobleaching of fluorescence from honey is observed. For longan honey and lichi honey, reduction of fluorescence is calculated to be 30% and 25% respectively, and the profile of fluorescence emission is unchanged. For schefflera honey, reduction of fluorescence from Em 363 is calculated to be 54%. Moreover, the component of Em 363 is degraded and its emission peak is shifted to 397 nm. Above all, fluorescence from honey exhibit good stability towards metal ions and heat treatment. In addition, protection from photo irradiation should be considered to avoid photobleaching and photodegradation.
出处
《蜜蜂杂志》
2015年第10期1-4,共4页
Journal of Bee
基金
福建省自然科学基金(2011J05042
2012J05037)
福建农林大学大学生创新训练项目(201510389201)
国家自然科学基金(NO.31201861)
现代农业产业技术体系(CARS-45-KXJ19)
关键词
蜂蜜
荧光
金属离子
热处理
光漂白
honeys
fluorescence
metalions
heat treatment
photobleaching
