摘要
目的:以雄性Sprague-Dawley(SD)大鼠为对象,探讨日粮中添加不同剂量的脱氢表雄酮(dehydroepiandrosterone,DHEA)对大鼠脂肪代谢常规生化指标及相关基因表达的影响。方法:将60只雄性SD大鼠随机分为对照组,DHEA低、中、高剂量组,各处理组大鼠分别灌胃0、25、50、100 mg/(kg·d)的DHEA(以体质量计,下同),连续灌胃8周,测定大鼠血脂、肝脂水平及肝脏脂肪代谢相关基因表达量。结果:DHEA中剂量组大鼠体质量显著低于对照组(P<0.05)。中剂量的DHEA可极显著降低大鼠血清中甘油三酯和血糖含量(P<0.01),低、中剂量的DHEA可显著升高大鼠血清高密度脂蛋白胆固醇含量(P<0.05)。脂肪代谢合成相关基因分析结果表明,低、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中脂肪酸合成酶(fatty acid synthase,FAS)m RNA表达水平(P<0.05或P<0.01);与对照组相比,不同剂量的DHEA均可显著或极显著降低大鼠肝脏组织中固醇调节元件结合蛋白-1(sterol regulatory element binding protein-1,SREBP-1)m RNA和肝细胞核因子-4(hepatocyte nuclear factor-4,HNF-4)m RNA表达水平(P<0.05或P<0.01),但对乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,ACC)m RNA表达水平均无显著影响(P>0.05)。脂肪代谢分解相关基因分析结果表明,不同剂量DHEA均可显著或极显著降低大鼠肝脏组织中酰基辅酶A氧化酶(acyl coenzyme A oxidase,ACO)m RNA表达水平(P<0.05或P<0.01);与对照组相比,低、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中肉毒碱棕榈酰转移酶-1(carnitine palmitoyl transterase-1,LCPT-1)m RNA和过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptor-α,PPARα)m RNA表达水平(P<0.05或P<0.01);中、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中脂肪三酰甘油脂肪酶(adipose triglyceride lipase,ATGL)m RNA表达水平(P<0.05或P<0.01)。结论:长期灌胃DHEA可影响雄性大鼠脂肪代谢途径中关键因子基因的�
This study aimed to investigate the effects of dehydroepiandrosterone （DHEA） on routine biochemical indicators and the expression levels of lipid metabolism-related genes. A total of 60 male Sprague-Dawley （SD） male rats were randomly divided into 4 groups： control group （0 mg/（kg·d） DHEA）, low-dose group （25 mg/（kg·d） DHEA）, moderate-dose group （50 mg/（kg·d） DHEA） and high-dose group （100 mg/（kg-d） DHEA）. The results showed that DHEA significantly decreased body weight in moderate-dose group when compared with control group （P 〈 0.05）. The levels of serum triacylglycerol and glucose were highly significantly decreased in moderate-dose group （P 〈 0.01）. The level of high-density lipoprotein cholesterol （HDL-C） was significantly increased in high-dose group （P 〈 0.05）. The results of quantitative real- time polymerase chain reaction （qRT-PCR） showed that mRNA expression level of fatty acid synthase （FAS） was significantly decreased in moderate-dose group （P 〈 0.05 or P 〈 0.01） when compared with control group. The mRNA expression levels of sterol regulatory element binding protein-1 （SREBP-1） and hepatocyte nuclear factor-4 （HNF-4） were significantly decreased in all the DHEA groups （P 〈 0.05 or P 〈 0.01）. However, the mRNA expression level of acetyl coenzyme A carboxylase （ACC） was not significantly changed （P 〉 0.05）. As for the adipolysis-related genes, the mRNA expression levels of acyl coenzyme A oxidase （ACO） were significantly decreased in all the DHEA groups （P 〈 0.05 or P 〈 0.01）. The mRNA expression levels of carnitine palmitoyl transterase-1 （LCPT-1） and peroxisome proliferator activated receptor-α （PPARα） were significantly decreased in both low- and high-dose groups （P 〈 0.05 or P 〈 0.01）. The mRNA expression levels of adipose triglyceride lipase （ATGL） were significantly decreased in both moderate- and high-dose groups （P 〈 0,05 or P 〈 0.01）. These fin
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第17期252-258,共7页
Food Science
关键词
脱氢表雄酮
脂类代谢
MRNA
dehydroepiandrosterone （DHEA）
lipid metabolism
mRNA
