摘要
目的:研究酒精性脂肪肝大鼠模型肝组织中HIFs/PPAR信号通路激活程度与脂质代谢的关系。方法:选择成年SD大鼠,通过酒精灌胃和高脂饮食的方式建立酒精性脂肪肝大鼠模型,取肝脏组织检测HIF-1α、PPARγ以及脂质代谢相关酶的含量,取血清检测脂质代谢指标和肝细胞损伤指标的含量。结果:(1)模型建立后1周、2周、3周和4周时,模型组大鼠肝脏中HIF-1α呈升高趋势、PPARγ呈降低趋势,且HIF-1α含量高于对照组、PPARγ含量低于对照组(P<0.05);(2)模型组血清中apoCII、apoCIII、α-GST、GLDH含量以及肝脏组织中FAT、FABP1、FAS、ACC、ACAT-2含量均显著升高(P<0.05),且与HIF-1α呈正相关、与PPARγ呈负相关。结论:酒精性脂肪肝大鼠模型肝组织中转录因子HIF-1α含量异常升高、PPARγ含量异常降低,能够通过增加肝脏中脂质代谢相关酶的表达来造成脂质代谢异常、肝细胞损伤。
Objective： To study the correlation of HIFs/PPAR signaling pathway activation degree and lipid metabohsm in liver tissue of alcoholic fatty liver rat model. Methods： Adult SD rats were selected and alcoholic fatty liver rat models were established by alcohol administration and high-fat diet feeding. Liver tissue was collected and contents of HIF-1a, PPARγ and lipid metabolism-related enzymes were detected; serum was collected and contents of lipid metabolism indexes and liver cell damage indexes were detected. Results, （1） one week, two weeks, three weeks and four weeks after models were established, HIF-1α in livers of model group showed an increasing trend and PPAR7 showed a decreasing trend; HIF-1α content was higher than that of control group and PPARγ content was lower than that of control group; （2） contents of apoCII, apoCIII, α-GST and GLDH in serum as well as levels of FAT, FABP1, FAS, ACC and ACAT-2 in liver tissue of model group all significantly increased, and were positively correlated with HIF-1α and negatively correlated with PPARγ. Conclusion, Transcription factor HIF-1α content abnormally increases and PPARγ content abnormally decreases in liver tissue of alcoholic fatty liver rat models; it results in abnormal lipid metabolism and liver cell damage through increasing the expression of lipid metabolism-related enzymes in the liver.
出处
《海南医学院学报》
CAS
2015年第11期1459-1462,共4页
Journal of Hainan Medical University
基金
陕西科学技术发展计划项目(2014K11-03-03-15)~~
