摘要
目的探究白细胞介素-22(IL-22)及IL-22R1、信号转导和转录激活因子-3(STAT3)、原癌基因(c-myc)在银屑病表皮异常增殖中的作用。方法采用逆转录聚合酶链反应(RT-PCR)技术对23例寻常型银屑病患者皮损和11例正常人皮肤中IL-22、IL-22R1、STAT3、c-myc的mRNA水平进行检测。结果 IL-22、IL-22R1、STAT3、c-myc的mRNA在寻常型银屑病皮损中和正常人皮肤中均呈阳性表达;但在寻常型银屑病皮损中的表达水平较正常人组明显升高,差异有统计学意义(P<0.01)。IL-22的mRNA水平与IL-22R1、STAT3的mRNA水平呈正相关(P<0.05);STAT3的mRNA水平与c-myc的mRNA水平呈正相关(P<0.05)。结论 IL-22可能诱导STAT3活化导致银屑病角质形成细胞(KC)过度增殖,这为银屑病治疗提供了新的靶点。
Objective To investigate the roles of IL-22,IL-22R1,Stat3 and c-myc mRNA expressions in abnormal proliferation of keratinocytes in psoriasis vulgaris.Methods Biopsies of skin lesions were conducted in 23 patients with psoriasis vulgaris and the skin samples of 11 normal control subjects.Lev-els of IL-22,IL-22R1,Stat3 and c-myc mRNA expressions were measured with reverse transcription-poly-merase chain reaction （RT-PCR）.Results IL-22,IL-22R1,Stat3 and c-myc mRNA were positively expressed in the psoriatic skin lesions as well as the normal controls.But the levels in the psoriatic skin lesions were significantly higher than those in the normal controls （P 〈0.01）.There were positive corre-lations between the expression of IL-22 mRNA and that of IL-22R1 /Stat3 mRNA （P 〈0.05 ）.There were also positive correlations between the expression of Stat3 mRNA and that of c-myc mRNA （P 〈0.05）.Conclusion The activation of Stat3 induced by IL-22 may lead to excessive proliferation of ke-ratinocytes in psoriasis vulgaris,which could provide a new perspective in the treatment.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2015年第8期529-531,555,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.81273755)
国家青年自然科学基金项目(No.81403404)
