摘要
目的 介绍一种新型复合纳米凝胶负载入胎盘素提取物过程.方法 取10 mg异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸（IKVAV）、自组装多肽分子（RAD16）、FGL多肽两亲性分子（FGL-PA）、精氨酸、甘氨酸和天冬氨酸合成多肽（RGD）联合制作理想纳米凝胶载体,纳米纤维凝胶稀释O、50、100倍后检测实验过程.结果 纳米纤维凝胶的最佳配比：IKVAV∶ RGD∶ FGL-PA=1∶1∶1.人胎盘提取液纳米纤维凝胶的平均粒径为212.8 nm,缓释时间达4d以上.包裹人胎盘提取液纳米纤维中人胎盘提取液和原料药结构保持基本完整.结论这种新型纳米复合材料极可能为人胎盘素提取物提供理想的药物运载系统.
Objective To describe a novel nanocomposite gel fiber encapsulated human placental extract through layer-by-layer self-assembly.Methods The 10 mg of isoleucine-lysine-valine-alanine-valine（IKVAV）,Arg-Gly-Asp （RGD） and FGL peptide-amphiphile （FGL-PA） were screened and combined to produce an optimal vehicle nanofiber gel through layer-by-layer assembly.We detected its morphology,particle size,drug loading efficacy,the encapsulation rate,release efficiency and the structure validation after it was diluted 0,50,100 times.Results The optimal proportion and concentration of nanometer fiber gel：IKVAV∶ RGD∶ FGL-PA =1 ∶ 1 ∶ 1,and 0.6 g/L by observing the liquidity,diffusion time and viscosity.The nanometer fiber gel had slow-releasing profile,its average particle size was 212.8 nm,and the slow release time was more than 4 days.The preliminary results confirmed that the structure of human placenta extract （HPE） encapsulated nanofiber gel was unvaried compared with the HPE structure.Conclusion This kind of novel composite nanocapsules may offer a promising delivery system for human placenta extract.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第9期2228-2230,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81271968）
