摘要
目的 分析上皮源性卵巢癌（EOC）组织中mi R-200a、mi R-141、mi R-205和mi R-34a的表达及其临床意义。方法 44例EOC患者按FIGO分期分为FIGOⅠ~Ⅱ（FIGO早期组）组15例和FIGOⅢ~Ⅳ（FIGO晚期组）组29例,用实时荧光定量PCR定量比较2组4种mi RNAs的表达差异并考察4种mi RNAs表达间的关联。以各组mi RNAs表达的中位数将EOC患者分为mi RNA高表达组和低表达组,比较2组患者间的生存差异。按年龄、FIGO分期、术后是否有残余瘤及术后化疗情况分组,进行Kaplan-Meier生存分析和Cox多因素分析。结果 FIGO晚期组mi R-141的表达高于早期组（P=0.036）。mi R-141的表达与mi R-200a、mi R-205呈正相关,与mi R-34a呈负相关（均P〈0.05）;mi R-200a与mi R-205的表达呈正相关（P〈0.05）。生存分析显示,mi R-200a的表达低,卵巢癌患者无进展生存期短（P=0.035）,FIGO早期组生存率高于FIGO晚期组（P〈0.05）。mi R-200a的表达水平、FIGO分期以及年龄与卵巢癌患者总生存期和无进展生存期有关,mi R-141、mi R-205和mi R-34a的表达水平、术后残余瘤以及化疗与患者生存无关。结论 mi R-200a的表达与卵巢癌患者预后有关,可能是卵巢癌患者预后预测的独立影响指标。
Objective To determine the expressions of miR-200a, miR-141, miR-205 and miR-34a in epithelial ovarian cancer （EOC） samples and to explore their clinical significance. Methods According to FIGO staging, 44 EOC patients were divided into two groups：early FIGO stage （stageⅠ-Ⅱ, n=15） and late FIGO stage （stageⅢ-Ⅳ, n=29）. Expressions of 4 miRNAs were detected by real time quantitative PCR, and were compared between two groups. The correlation of 4 miRNAs was calculated. EOC patients were divided into high miRNA expression group and low expression group according to the median value of miRNAs expression. Kaplan-Meier survival analysis and Cox multivariate analysis were used to compare the age, FIGO state, tumor residual after operation and post-operative chemotherapy of ovarian cancer between two groups. Results The expression of miR-141 was elevated in stagesⅢandⅣcompared with that of stagesⅠand Ⅱ（P=0.036）. There was a positive correlation between expression of miR-141, miR-200a and miR-205, but a negative correlation with miR-34a （P〈0.05）. There was a positive correlation between miR-200a and miR-205 （P〈0.05）. Lower miR-200a expression was associated with shorter progress free survival in ovarian cancer analyzed by log-rank test （ P=0.035）. The survival rate was significantly higher in FIGO stages ⅠandⅡthan that of FIGO stagesⅢandⅣ（P〈0.05）. Cox regression analysis revealed that miR-200a, FIGO stage and age were influential factors of overall survival time and progress-free survival time of ovarian cancer, while miR-141, miR-205, miR-34a and tumor residual after operation and post-operative chemotherapy were not influential factors. Conclusion The expression of miR-200a is closely correlated with the progress and prognosis of ovarian cancer and may be used as an independent indicator for ovarian cancer prognosis.
出处
《天津医药》
CAS
2015年第9期996-1000,共5页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(81072363)
