摘要
利用ISSR分子标记技术对我国不同地区收集的金线兰及其近缘种种质资源进行亲缘关系及遗传多样性分析。结果显示：9条ISSR引物在50份材料中共扩增条带156条,其中多态性条带155条,多态性比率达99.36%;UPGMA树状图分析显示,样本总体相似系数介于0.56~0.90之间,在相似系数0.65处可分4大类群,其中金线兰主要集中于A大类群。A大类群又可分为3个亚类5个小亚类,亚类中不同地区来源的金线兰种质没有明显的界限。去除部分地区来源不明材料,46份材料依不同地理来源分成8个种群,其中福建野生金线兰4个种群,二者Nei＇s基因多样性指数（H）分别为0.322和0.295,处兰科植物中等水平;种群遗传分化系数（Gst）分别为0.328和0.240,处兰科植物较低水平。AMOVA分析显示样本种群内变异指数87%,种群间变异指数13%,表明种群间可能存在基因交流。
ISSR molecular markers were employed to assess the genetic diversity and differentiation of Anoec- tochilus roxburghii and it＇s relative species collected from different districts of China. The results indicated that, a to- tal of 156 bands were amplified from 50 samples by 9 ISSR primers, and 155 were polymorphic, the percentage of polymorphic bands was 99. 36%. UPMGA dendrogram analysis indicated that, the similarity coefficient ranged from 0. 56 to 0. 90, at genetic similarity of 0. 65, all the 50 samples were clustered into 4 groups, and A. roxburghii were mainly gathered in group A. Group A can further clustered into 3 subgroups and 5 small subgroups, and there were no significantly boundaries among A. roxburghii samples collected from different districts. After removed unknown source samples, based on geographic source ,46 samples were divided into 8 populations, included 4 Fujian wild A. roxburghii populations, the values of Nei＇s gene diversity （H） of them were 0. 322 and 0. 295, which were in average level compared with other orchids ; the coefficient of genetic differentiation among population （Gst） were 0. 328 and 0. 240, which were in low level compared with other orchids. Results of AMOVA analysis indicated that genetic variation within population was 87%, variation among population was 13%, indicated there could exist gene flow among populations.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2015年第5期1045-1054,共10页
Journal of Plant Genetic Resources
基金
福建省自然科学基金项目(2014J01382)
三明市农业科学研究院院立项目(2014-Y-05)
关键词
金线兰
分子标记
基因多样性
ISSR
Anoectochilus roxburghii
molecular maker
genetic diversity
ISSR
