摘要
目的观察更年安神方对围绝经期(Perimenopausal syndrome,PMS)大鼠下丘脑雌激素受体(Estrogen receptor,ER)和血清细胞因子的影响,探讨更年安神方改善围绝经期失眠等症状的可能机制。方法通过阴道涂片筛选出自然衰老的围绝经期大鼠,随机分为围绝经期对照组、中药更年安神方组;另取5月龄雌性大鼠作为青年对照组。各组大鼠分别连续灌胃给予蒸馏水、中药更年安神方、蒸馏水4周。采用酶联免疫吸附法测定围绝经期大鼠经中药更年安神方治疗后,下丘脑雌激素受体水平和血清TNF-α的变化。结果中药更年安神方组较围绝经期组大鼠下丘脑ER水平有所增多,增幅尚未达到统计学差异。更年安神方组血清TNF-α高于围绝经期对照组(P<0.05)。结论更年安神方对于围绝经期睡眠的调节可能与调节血清TNF-α的含量有关。
OBJECTIVE To observe the effects of Geng-Nian-An-Shen-Fang on the expression of estrogen re-captor in hypothalamus of rats during perimenopausal period.METHODS The rat model of perimenopausal syn-drome was established by unforced aging.The Wistar rats were randomly divided into two groups：perimenopausal pe-riod controls,Geng-Nian-An-Shen-Fang group.The 5-month old female rats served as young controls.After the treat-ment of Geng-Nian-An-Shen-Fang in Perimenopause rats,levers of ER in hypothalamus were measured by enzyme linked immunosorbent assay.RESULTS Compared with perimenopausal period group,the expressions of ER in hy-pothalamus of Chinese medicine group increased （ P＆gt;0.05 ） .Significant differences in serum level of TNF-αwere observed between Geng-Nian-An-Shen-Fang group and perimenopausal period group（ P＆lt;0.05）.CONCLUSION Geng-Nian-An-Shen-Fang in improving sleep quality may be based on its regulation of serum level of TNF-α.
出处
《海峡药学》
2015年第8期23-25,共3页
Strait Pharmaceutical Journal
基金
福建省中青年教师教育科研项目(科技B类)(项目编号:JB13091)
福建省卫生厅青年科研课题项目(项目编号:2013-2-72)
关键词
更年安神方
围绝经期
下丘脑
雌激素受体
血清细胞因子
Geng-Nian-An-Shen-Fang
Perimenopausal syndrome
Hypothalamus
Estrogen receptor
Serum Cytokine
