摘要
目的评价XE-5000全自动血液细胞分析仪检测外周血有核红细胞（NRBC）的主要性能特点,并探讨其临床应用价值。方法对XE-5000全自动血液细胞分析仪的主要性能进行检测,并选取137份经分析仪DIFF通道检测提示NRBC阳性的患者血液标本,采用分析仪NRBC通道检测NRBC计数和百分比,采用显微镜分类检测NRBC百分比,采用SPSS18.0统计软件进行统计分析。结果高、中和低值标本的NRBC计数及NRBC百分比的批内精密度分别为2.10%、3.26%、11.62%和3.79%、5.80%、13.33%,携带污染率NRBC计数为0.51%,NRBC百分比为0.26%,在（0～18）×109/L范围内XE-5000全自动血液细胞分析仪对NRBC的检测结果呈良好的线性：Y=1.048 6 X＋0.189 6（r=0.999 1）。XE-5000全自动血液细胞分析仪与显微镜分类计数法的NRBC百分比检测结果比较差异无统计学意义（P=0.716）,呈良好的相关性：Y=1.150 2 X＋0.626 1（r=0.967 0）。结论 XE-5000全自动血液细胞分析仪可替代传统的显微镜分类计数方法应用于临床。
Objective To evaluate the performance of XE‐5000 automated blood cell analyser for detecting nucleated red blood cell （NRBC） in peripheral blood and investigate its clinical application value .Methods The intra‐assay imprecision ,carryover rate , and linear range of the analyser were evaluated .The absolute NRBC count and percentage of NRBC of 137 blood specimens （NRBC‐positive according to the DIFF channel of the analyser） were determined in the NRBC channel of the analyser ,and the percentage of NRBC of these blood specimens were determined by using microscope method as well .Differences between the two methods were analysed by using SPSS18 .0 statistic software .Results The intra‐assay imprecision of the analyser for detecting absolute NRBC count in specimens with high ,moderate ,and low Q‐flag values were 2 .10% ,3 .26% and 11 .62% ,respectively ,and the imprecision for detecting percentage of NRBC were 3 .79% ,5 .80% and 13 .33% ,respectively .The carryover rates of the analyser for detecting absolute NRBC count and percentage of NRBC were 0 .51% and 0 .26% ,respectively .At the range of （0～18）×109/L ,the absolute NRBC count detected by using the analyser showed good linearity ：Y=1 .048 6X＋0 .189 6（r=0 .999 1） .There were no statistically significant differences between the analyser and microscope for detecting percentage of NRBC（P=0 .716） ,and showed good corre‐lation：Y=1 .150 2X＋0 .626 1（r=0 .967 0） .Conclusion The XE‐5000 automated blood cell analyser could completely replace the traditional microscope for clinically classifying and counting NRBCs .
出处
《国际检验医学杂志》
CAS
2015年第18期2666-2668,2671,共4页
International Journal of Laboratory Medicine
关键词
血液细胞分析仪
有核红细胞
性能
外周血
blood cell analyzer
nucleated red blood cell
performance
peripheral blood
