摘要
为提高黑萨福克绵羊原核期胚胎的生产效率,通过CIDR＋FSH＋PMSG和CIDR＋FSH＋PG两种激素方案对供体进行超数排卵,将冲出的胚胎移植入同期发情处理的受体。其中CIDR＋FSH＋PMSG注射组供体平均黄体数、可用胚数和移植妊娠率均高于CIDR＋FSH＋PG注射组（P〈0.05）。同时,在CIDR＋FSH＋PMSG注射组,不同FSH剂量注射对黑萨福克绵羊超数排卵有影响。在180-240 IU剂量组,供体羊的平均黄体数、可用胚胎数高于另一组。繁殖季节（秋季）供体平均黄体数、可用胚胎数均显著高于春季（P〈0.05）。
The objective of this study was to improve the efficiency of Black Suffolk pronuclear -stage embryo yield to be used for DNA microinjection in a transgenesis program.The Black Suffolk sheep was superovulated by CIDR ＋PMSG ＋FSH and CIDR ＋FSH ＋PG. Then the flushed out embryo was transferred to the estrus synchronized recipient by speculum insemination＋nature mating.In the CIDR＋FSH＋PMSG injection group, the average number of corpus luteum, available embryo and pregnancy rate from the Black Suffolk were significantly higher compared to the CIDR＋FSH＋PG injection group （ P〈0.05 ） . Meanwhile, different dose of FSH injection had a impact on the Black Suffolk sheep superovulation.When the FSH injection dose was during 180 -240 IU, the average number of corpus luteum and available embryo from the Black Suffolk sheep were 15.87 and 13.88, respectively.The average number of corpus luteum and available embryo were significantly higher in the breeding season （ autumn） compared to spring（ P〈0.05） .
出处
《黑龙江动物繁殖》
2015年第5期6-10,共5页
Heilongjiang journal of animal reproduction
基金
国家科技支撑项目(2011BAD19B02-4)
转基因重大专项(2014ZX08008-002B
2011ZX08008-005)
关键词
黑萨福克
超数排卵
原核胚
Black Suffolk
pronuclear embryo
superovulation
