摘要
【目的】观察丹参酮ⅡA对胃癌细胞增殖的影响及其与环氧合酶-2（COX-2）表达的关系。【方法】体外培养胃癌细胞株SGC7901，经丹参酮ⅡA作用后，采用四唑盐（MTT）比色法检测细胞增殖，流式细胞仪检测细胞凋亡，免疫细胞化学sABC法检测COX-2蛋白表达，放射免疫法检测前列腺素E2（PGE2）含量。【结果】丹参酮ⅡA对胃癌细胞的生长有明显的抑制作用，并呈剂量依赖性。以0．5mg／L作用终浓度抑制作用最明显，其48h的抑制率为71．1％，与对照组相比差异有显著性（P〈0．01）；5mg／L丹参酮ⅡA作用后，随时间的延长，凋亡率逐渐升高，48h达到高峰，随后逐渐下降，与对照组比较，各处理组都有显著性差异（P〈0．05）；丹参酮作用组胃癌细胞COX-2表达明显减少，其培养液中PGE2的产生量下降，与对照组相比差异均有显著性（P〈0．01）。【结论】丹参酮ⅡA可能是通过下调COX-2mRNA的表达水平发挥其对胃癌细胞生长抑制及促进凋亡作用。
[ObiectivelTo observe the effect of TanshinonellA on proliferation and apoptosis of human gastric carci noma cell line SGC7901 and its correlation with COX-2 expression.[Methods]Human gastric carcinoma cell line SGC7901 was cultured in vitro and treated with Tanshinone HA. The cell proliferation was detected by MTT assay, and the cell ap- optosis was detected by flow cytometry, the expression of COX-2 protein was detected by immunohistochemistry SABC method, and the content of prostaglandin E2 （PGE2） was detected by radioimmunoassay.[Results]The growth of gastric cancer cells was significantly inhibited by TanshinoneⅡA , and dose dependence there occurred. The inhibition effect of 0.5 mg/L was the most obvious dosage, and the inhibition rate of 48 h reached 71.1%, the difference was significant com- pared with the control group （ P 〈0.01） ; With 5 mg/L of TanshinonelIA trestment , the apoptosis rate increased grad ually with the time and reached its peak at 48th h, then gradually decreased. Compared with the control group, the treat- ment group had significant difference （ P 〈0.05） ; COX-2 expression in gastric cancer cells of the treatment group was significantly decreased, and the production of PGE2 in the culture fluid decreased significantly （ P 〈0.01）.[Conclusion] TanshinoneUA may play a role in the growth inhibition and apoptosis of gastric cancer cells by down regulating the expres- sion of COX-2 mRNA.
出处
《医学临床研究》
CAS
2015年第8期1514-1517,共4页
Journal of Clinical Research
