摘要
目的 胃癌可异位分泌绒毛膜促性腺激素 (hCG) ,且与胃癌的恶性程度及转移有关。探讨hCG对人胃腺癌细胞胞浆信号传递的影响。方法 分别用非同位素检测试剂盒、蛇毒磷酸二酯酶法、3 H cAMP放射免疫法 ,观察hCG对胃腺癌SGC 790 1细胞胞浆内蛋白激酶 (PKC)、钙调素 (CaM )活性及cAMP含量的影响情况。结果 hCG对胞浆PKC活性仅有轻微的刺激作用 ,即当hCG的浓度达10 -6mol/L时 ,细胞内PKC活性与对照相比仅增加了 2倍左右 ;hCG可明显使胞浆内cAMP含量及CaM活性增加 ,当hCG的浓度从 10 -11mol/L增加到 10 -6mol/L时 ,胞内cAMP的水平从 (2 .1± 0 .2 5 )pmol/mg蛋白增至 (9.5 2± 0 .6 4 ) pmol/mg蛋白 (P <0 .0 1) ,而CaM含量由 (11.2± 2 .7)μg/mg蛋白增加到 (2 9.3± 3.4 2 ) μg/mg蛋白 (P <0 .0 1) ,与对照组比较差异均有显著性 (P <0 .0 1)。 结论 hCG作用于胃腺癌细胞后可能主要通过激活腺苷酸环化酶 (AC) /cAMP信号传递途径而起作用 ,同时Ca2 + /CaM可能亦参与了其信号传递机制。
Objective Human chorionic gonadotropin(hCG), which could be ectopic secreted in patients with gastric cancer, was related to the malignant degree and metastasis of cancer. The present study was to investigate the effects of hCG on the intracellular signal transduction in human gastric adenocarcinoma cells(SGC 7901). Methods The effects of hCG on intracellular protein kinase C(PKC) and calmodulin(CaM) activities and cAMP levels in SGC 7901 were studied with non radioactive detection kit, phosphodiesterase(PDE) assay and radioimmunoassay methods, respectively. Results As the concentration of hCG increased from 10 -11 mol/L to 10 -6 mol/L, cellular PKC activity in SGC 7901 cells only increased by about 2 folds, but cellular cAMP levels and CaM content increased from (2.1±0.25) pmol/mg protein to (9.52±0.64) pmol/mg protein and from (11.2±2.7) μg/mg protein to (29.3±3.42) μg/mg protein, respectively, these were significant differences compared with controls( P <0.01). Conclusions hCG exerts its effects on gastric cancer cells possibly by activating adenyl cyclase(AC)/cAMP signal transduction pathway and the Ca 2+ /CaM was also involved in its signaling transduction.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第8期470-473,共4页
Chinese Journal of Digestion
