摘要
目的研究克白颗粒抗白癜风的体内外药效,并对克白颗粒进行安全性评价。方法采用血清药理学实验方法检测克白颗粒含药血清对小鼠黑色素瘤细胞B16f10增殖能力的影响;选取50只健康豚鼠随机分成正常对照组、模型对照组及克白颗粒高、中、低剂量(9.04,4.52,2.26 g·kg?1·d?1)组,每组10只,除正常对照组外,其余各组采用化学脱色法连续涂抹7%H2O2 50 d,制备实验性白癜风豚鼠模型,肉眼观察各给药组对实验性白癜风豚鼠模型的疗效,检测豚鼠血液中酪氨酸酶(TRY)、胆碱酯酶(CHE)、单胺氧化酶(MAO)、丙二醛(MDA)的含量;长期毒性实验对克白颗粒进行安全性评价。结果血清药理学实验表明克白颗粒含药血清能够显著促进B16f10细胞的体外增殖。造模后,模型组豚鼠血清MDA、MAO含量显著升高(P<0.05),CHE、TRY含量显著降低(P<0.05或0.01);与模型对照组比较,克白颗粒高剂量显著降低血清中MDA含量(P<0.01),高、中剂量显著降低MAO的含量(P<0.05)、显著升高CHE的含量(P<0.05),但对TRY含量无显著影响。长期毒性实验结果显示克白颗粒对SD大鼠脏器重量无显著影响。结论克白颗粒显示出良好的抗白癜风效果,其作用机制可能与调节血液中CHE、MAO、MDA含量、促进黑色素细胞增殖有关。
OBJECTIVE To explore the anti-vitiligo effects of Kebai particles in vitro and in vivo and evaluate the safety of Kebai particles. METHODS The effects of Kebai particles on mouse melanoma cells B16f10 proliferation in vitro was tested by serum pharmacology method. Long term toxicity test were carried out to evaluate the safety of Kebai particles. 50 guinea pigs were randomly divided into 5 groups： normal control group, model control group, high, middle and low dose group of Kebai particles（9.04,4.52,2.26 g·kg^-1·d^-1）. The experimental vitiligo guinea pig model was prepared by 7% H2O2 for 50 d. Melanin distribution in skin and cholinesterase（CHE）, monoamine oxidase（MAO）, malondialdehyde（MDA） and tyrosinase（TYR） in blood were determined. RESULTS The results of serum pharmacology experiment showed that medicated serum significantly promoted the proliferation of B16f10 cells in vitro. Long-term toxicity experiment results indicated Kebai particles exhibited no effect on viscera weight of SD rats. Animal experimental data showed that the serum level of MAO and MDA significantly increased in model control group（P〈0.05）, while the serum level of CHE and TRY significantly decreased in model control group（P〈0.01 or 0.05）. Compared with the model control group, high dose of Kebai particles significantly decreased the serum content of MDA（P〈0.01）, high and middle dose of Kebai particles significantly decreased the content of MAO and increased the content of CHE（P〈0.05）. CONCLUSION Kebai particles show good anti-vitiligo effects, its main mechanism may be related to decrease the contents of serum CHE, MAO and MDA and promote the proliferation of melanocytes.
出处
《中国现代应用药学》
CAS
CSCD
2015年第9期1046-1050,共5页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省中西医结合学会科研基金项目(2013LYZD019)
关键词
克白颗粒
白癜风
血清药理学
动物模型
Kebai particles
vitiligo
serum pharmacology methods
animal model
