摘要
目的探讨人附睾上皮分泌蛋白4(HE4)对子宫内膜癌的诊断意义,及其与糖链抗原125(CA125)联合检测对子宫内膜恶性肿瘤与子宫内膜良性病变的诊断价值。方法分别应用电化学发光法和双抗体夹心时间分辨免疫荧光分析法检测26例子宫内膜癌患者、28例功能失调性子宫出血患者及32例健康女性血清中的HE4和CA125水平,并进行分析。结果子宫内膜癌组的HE4水平明显高于功能失调性子宫出血组和健康对照组,差异均具有统计学意义(t值分别为2.463、2.471,均P<0.05);子宫内膜癌组中,单独检测HE4的灵敏度和特异度分别为46.27%和95.08%,单独检测CA125的灵敏度和特异度分别为39.91%和98.21%,二者联合检测时灵敏度和特异度分别为52.73%和95.03%,较CA125和HE4单项检测灵敏度增高,特异度较CA125略低。结论联合检测HE4和CA125提高了检测灵敏度,特异度降低,对子宫内膜癌具有更高的诊断价值,为联合检测HE4和CA125鉴别子宫内膜良、恶性肿瘤提供临床依据。
Objective To explore the diagnostic value of human epididymis secretory protein 4 （ HE4） in endometrial malignant tumor and to compare it with carbohydrate antigen 125 （ CA125 ） in diagnosis of endometrial malignant tumor and endometrial benign lesions. Methods Serum levels of HE4 and CA125 in 26 cases of endometrial carcinoma, 28 cases of dysfunctional uterine bleeding, and 32 cases of healthy controls were detected with electrochemiluminescence immunoassay and double antibody sandwich time-resolved immunofluorescence analysis method.Results Serum levels of HE4 among endometrial cancer patients were obviously higher than those of patients with dysfunctional uterine bleeding and healthy controls, and the differences were significant （ t value was 2.463 and 2.471, respectively, both P〈0.05）.In endometrial cancer group, the sensitivity and specificity of detection of HE4 were 46.27%and 95.08%, respectively, and those of CA125 were 39.91%and 98.21%, respectively.The sensitivity of joint detection （52.73%） was higher than that of HE4 and CA125, but the specificity of joint detection （95.03%） was lower than that of CA125.Conclusion Joint detection of HE4 and CA125 improves the sensitivity but reduces the specificity, which has higher diagnostic value for endometrial cancer.It provides clinical evidence for differential diagnosis of endometrial benign from malignant tumors.
出处
《中国妇幼健康研究》
2015年第4期709-711,714,共4页
Chinese Journal of Woman and Child Health Research
关键词
子宫内膜恶性肿瘤
子宫内膜良性病变
人附睾上皮分泌蛋白4
糖链抗原125
endometrial malignant tumor
endometrial benign lesions
human epididymis secretory protein 4 （ HE4 ）
carbohydrate antigen125 （CA125）
