摘要
目的观察补肾活血汤(AED)对去卵巢大鼠心肌中细胞色素P4502J3蛋白(CYP2J3)表达、心肌微血管数目和血清雌二醇(E2)水平的影响。方法 48只成年SD雌鼠随机分为8组:正常对照组(Control组)、假手术组(Sham组)、去卵巢组(OVX组)、AED高剂量组(AED-H组)、AED低剂量组(AED-L组)、17β-雌二醇干预组(OVX/ERT组)、雌激素受体拮抗剂ICI182780干预组(AED-H/FET组及AED-L/FET组)。各组大鼠在处死前进行血液流变性指标的检测;Q-PCR和Western blot检测心肌CYP2J3的表达变化,免疫组化染色法检测心肌微血管数目变化,心脏B超检测心肌血流动力学变化,放射免疫法检测血清E2水平改变。结果 OVX组心肌微血管计数明显下降(P<0.01),而OVX/ERT组与AED-H组心肌微血管计数改善明显;OVX组大鼠心肌CYP2J3 mRNA和蛋白表达明显下降,而OVX/ERT组与AED-H组的CYP2J3 mRNA和蛋白表达下降不明显;OVX组大鼠的全血黏度、血浆黏度及红细胞压积较Sham组大鼠增高,而OVX/ERT组及AED-H组全血黏度、血浆黏度及红细胞压积明显下降。OVX组大鼠的红细胞变形指数、红细胞刚性指数和红细胞电泳时间均明显增高(P<0.01),但在OVX/ERT组及AED-H组较OVX组大鼠明显下降(P<0.05)。OVX组血流动力学各项指标均降低(P<0.05),而与补充雌激素组相同,AED-H组左室射血分数改善明显。OVX组大鼠血清E2含量显著下降(P<0.05),高剂量、低剂量AED均可使OVX组大鼠E2含量显著升高(P<0.05)。结论 AED能增加心肌微血管数目,改善心肌血流动力学,其作用可能与增加OVX大鼠循环雌激素水平及促进CYP2J3蛋白表达有关。
AIM To observe the effects of bushenhuoxue decoction on myocardial blood flow dynamics, number of myocardial microvascular vessels, serum estradiol levels and myocardial CYP2J3 expression in ovariectomized rats. METHODS Forty-eight adult female Sprague Dawley rats were randomly divided into eight groups： normal group （NORM） , sham group （SHAM） , ovariectomized group （OVX） , high- dose Bushenhuoxue decoction group （AED-H）, low-dose Busbenhuoxue decoction group （AED-L） , 17β-estradiol intervention group （OVX/ERT group）, and ICI182780 intervention groups （AED-H/FET and AED-L/FET）. Hemorheology was detected before rats were killed. Cardiac hemodynamic were detected by cardiac uhrasound instrument. The number of myocardial microvascular vessels was deter- mined by immunohistochemical method. Serum estradiol levels were measured by radioimmunoassay, and cardiac CYP2J3 expressions were determined by Q-PCR and Western blot. RESULTS Blood flow dynamic indicators declined in OVX group compared to those in NORM group （P 〈 0. 05）. Left ventricular ejection fraction improved significantly in both estrogen group and AED-H group. Serum E2 level decreased significantly in OVX group （P 〈 0.05 ） but both high- and low-dose Bushenhuoxue decoction significantly promoted the serum E2 level in OVX group （P 〈 0. 05 ）. In OVX group, whole blood viscosity, plasma viscosity and hematocrit increased compared to sham group, whereas in OVX/ERT group and AED-H group, these indexes decreased obviously. Red cell deformation time index, erythrocyte rigidity index and erythrocyte electrophoresis were significantly higher in the OVX group （P 〈 0. 01 ）. However, these inde- xes significantly decreased in OVX/ERT group and AED-H group compared to OVX group （P 〈 0. 05）. Myocardial microvascular counts in OVX group decreased significantly, whereas the myocardial microvascular count in estrogen group and AED-H group improved significantly （ P 〈 0. 05 ）. Similarly, myocardial CYP2J3 mRNA
出处
《心脏杂志》
CAS
2015年第5期540-546,共7页
Chinese Heart Journal
基金
陕西省科学技术研究发展计划项目资助(2012K19-05-07)
陕西省教育厅科学研究计划项目资助(14JK1190)
