摘要
目的研究靛玉红、色胺酮对脂多糖(LPS)诱导的RAW264.7炎症细胞的抗炎作用。方法以10μg/ml LPS刺激RAW264.7细胞24 h诱导UC体外炎症模型,以MTT法检测靛玉红、色胺酮的对正常细胞的作用,以酶联免疫吸附法(ELISA)研究靛玉红、色胺酮在直接干预、预防干预2种模式下对细胞培养液白细胞介素-6(IL-6)/肿瘤坏死因子(TNF-α)浓度的影响。结果模型组中IL-6/TNF-α浓度较正常组均有明显升高(P<0.01);靛玉红直接干预时,细胞培养液IL-6(5.0、10.0μmol/L)/TNF-α(2.5、5.0、10.0μmol/L)均较模型组有明显下降(P<0.05);色胺酮直接干预时,细胞培养液IL-6(0.8、8.0μmol/L)/TNF-α(0.8μmol/L)均较模型组有明显下降(P<0.01,P<0.05),预防作用模式下,细胞培养液IL-6(0.08、0.8μmol/L)较模型组有明显下降(P<0.05)。结论靛玉红、色胺酮有一定的体外抗炎作用,抗炎模式以直接干预为主,抗炎机制可能为下调IL-6/TNF-α的表达,可用于防治溃疡性结肠炎。
Objective To investigate anti - inflammatory effect of indirubin and tryptanthrin on LPS Induced RAW264. 7 inflammatory cell model. Methods Murine macrophageRAW264. 7 cells were stimula-ted by 10 μg/ ml LPS for 24 h to establish an UC inflammatory cell model. Cell viability was detected by MTT assay to explore the safe range of concentrations. UC inflammatory cell model was treated by indirubin and tryptanthrin in two different ways：treatment mode and prevention mode. IL - 6 and TNF - αin supernatant was tested by Elisa assay. Results IL - 6 and TNF - α in supernatant in model group were significantly higher than control group（P ﹤ 0. 01）;Under treatment mode,IL - 6（5. 0、10. 0 μmol/ L）and TNF - α（2. 5、5. 0、10. 0 μmol/ L）in supernatant were reduced by indirubin significantly（ P ﹤ 0. 05）;IL - 6（0. 8、8. 0μmol/ L）and TNF - α（0. 8 μmol/ L group）in supernatant were reduced by tryptanthrin significantly（P ﹤0. 01,P ﹤ 0. 05,P ﹤ 0. 01）;While under prevention mode,only IL - 6（0. 08、0. 8 μmol/ L groups）in super-natant were reduced by tryptanthrin（P ﹤ 0. 05）. Conclusion Indirubin and tryptanthrin could inhibit in-flammation in UC inflammatory cell model in vitro,mainly under treatment mode by down - regulating IL - 6 and TNF - αexpression.
出处
《世界中西医结合杂志》
2015年第8期1069-1072,共4页
World Journal of Integrated Traditional and Western Medicine
基金
北京中医药大学创新团队资助项目(No.2011-CXTD-24)
关键词
溃疡性结肠炎
青黛
RAW264.7
细胞因子
实验研究
Natural indigo effective components
Ulcerative colitis
RAW264. 7 cells
Cytokine
ex-periment research
