摘要
目的探讨HLA-DRBI、HLA-DQB1基因多态性与胃癌的关联。方法利用序列特异性引物-聚合酶链反应（PCR—SSP）技术对38例胃癌患者染色体进行HLA-DRB1、HLA-DQB1基因分型并进行基因平衡（Hardy-Weinberg）分析，对照组为200例健康个体，通过统计学方法比较两组数据各位点基因频率有无统计学意义。结果实验组HLA—DRB1、HLA—DQB1基因分布情况符合遗传规律（P〉0．05），HLA-DRB1＊09基因频率低于对照组，差异有统计学意义（P〈0．05），其余位点基因频率实验组与对照组无统计学意义。结论HLA-DRB1＊09可能为胃癌的抑制基因。
Objective To explore the association between gastric cancer and genetic polymorphism of HLA-DRB1 and HLA-DQB1. Methods Polymerase chain reaction sequence specific primer（PCR-SSP） was applied to analyze HLA-DRB1 and HLA-DQB1 genotypes in 38 patients with gastric cancer Hardy-Weinberg balance was tested 200 healthy people were collected as control group. The difference between the two groups in frequency of genes was analyzed. Results The gene distribution of HLA-DRB1 and HLA-DQB1 was consistent with hereditary rules（ P 〉 0.05 ）. The gene frequency of HLA-DRB1 ＊ 09 was significantly lower（ P 〈 0.05 ） than that in control group. There were no significant differences in the rest of gene loci between experimental group and control group. Conclusion HLA DRB1 ＊ 09 may be the inhibiting gene of gastric cancer.
出处
《国际免疫学杂志》
CAS
2015年第5期423-426,共4页
International Journal of Immunology
