摘要
采用Hummers方法制备氧化石墨,用绿色环保还原剂L-半胱氨酸还原氧化石墨制得石墨烯（GN）,GN溶解在0.1%的Nafion溶液中,得到高分散性的GN悬浮液,用电聚合方法将对氨基苯磺酸（PABSA）聚合到GN表面,得到对氨基苯磺酸/石墨烯/Nafion修饰电极（PABSA/GN/Nafion/GCE）,用差分脉冲伏安法研究了芦丁在此修饰电极的电化学行为。由于PABSA与GN的协同作用,芦丁的电化学响应比PABSA修饰电极和GN修饰电极明显增强,极大的提高了检测灵敏度,对溶液的p H和扫描速率等实验条件进行了优化处理。在最佳实验条件下,芦丁的峰电流值与其浓度在5×10-8～1×10-5mol/L呈良好的线性关系,检出限为2.25×10-8mol/L（3σ）。方法可用于市售复方芦丁片的测定,加标回收率为96.4%～99.3%。
Graphite oxide（GO） was synthesized by the Hummers method,the stable form of graphene（GN） was subsequently prepared by uhrasonicating GO in the presence of green and environmental reducing agent L cysteine. To get high dispersibility, GN was dissolved in 0. 1% of Nafion solution and designed as GN suspension. A PABSA/GN/Nafion/NGCE composite-modified electrode was fabricated by using a GN nanosheets modified glassy carbon electrode （GCE） electropolymerized with p-aminobenzene sulfonic acid （PABSA）. Differential pulse vohammetry （DPV） method was used to study electrochemical behavior of rutin at modified electrodes. It was found that the values of peak current at PABSA/GN/Nafion/GCE were obviously larger than those at bare GCE, PABSA electrode and GN modified electrode,which indicated that the sensitivity for detection of rutin was improved. Experimental parameters, including pH value of buffer solution and scan rate were optimized. Under the optimal conditions,the fabricated sensor displayed excellent analytical performance for rutin concentration from 5 × 10 -8 mol/L to 1 × 10 -5mol/L with a low detection limit of 2.25 × 10 -8mol/L at 3σ. The modified electrode was applied in the detection of rutin in rutin tablets with recoveries of 96.4% ～ 99.3%
出处
《分析试验室》
CAS
CSCD
北大核心
2015年第9期1045-1050,共6页
Chinese Journal of Analysis Laboratory
基金
山西省自然科学基金(2012011007-1)资助
