摘要
为探讨生防细菌SY286菌株液体发酵条件,提高其活性次级代谢产物的产量,以菌体生物量和发酵液抑制葡萄霜霉病菌(Plasmopara viticola)活性为指标,采用单因子试验和正交试验对菌株的最适发酵培养基成分及发酵条件进行优化。结果表明,菌株SY286最适发酵培养基为葡萄糖10 g/L、牛肉膏7.5 g/L、氯化钠2.5 g/L、硝酸钾5 g/L;最佳发酵培养条件为培养温度27℃、培养时间72 h、初始p H 7.0、250 m L三角瓶装液量90 m L、接种量体积分数5%、摇床转速150 r/min。在最佳发酵培养基和培养条件下,菌株SY286发酵液抑菌率达到99.19%,抑菌能力提高6.98%。
In order to investigate best condition for liquid fermentation of strain SY286 and increase itsantagonistic substance productivity against Plasmopara viticola, the single factor experiments and orthogonalexperiments were adopted to optimize the liquid fermentation medium and fermentation condition. The resultshowed that the optimum medium was glucose 10 g/L, beef extract 7.5 g/L, Na Cl 2.5 g/L, KNO3 5 g/L. Theoptimal fermentation conditions were culture temperature 27℃, fermentation time 72 h, initial p H 7.0, mediumcapacity 90 m L/250 m L, inoculation rate 6%, and rotate speed 150 r/min. The inhibitory activity of strainSY286 fermentation solution increased by 6.98% under the optimum fermentation medium and cultureconditions, it was up to 99.19%.
出处
《中国农学通报》
2015年第25期157-163,共7页
Chinese Agricultural Science Bulletin
基金
公益性行业(农业)科研专项"果树霜霉病防控技术研究与示范"(201203035)
葡萄育种及综合配套技术创新团队"优质果树新品种选育及综合配套栽培技术"(2014204004)
关键词
发酵条件优化
单因子试验
正交试验
葡萄霜霉病菌
fermentation condition optimization
single factor experiment
orthogonal experiment
Plasmopara viticola
