摘要
目的研究甘草次酸-壳聚糖纳米粒(GA-CS-NPs)的最佳制备处方并对其进行质量评价。方法采用离子交联法制备GA-CS-NPs,以粒径、载药量、包封率为综合评价指标,通过单因素、正交设计试验优化制备工艺及处方,通过形态观察、粒径、载药量及包封率的考察对其进行质量评价。结果最佳处方组合为甘草次酸(GA)质量浓度为0.2 mg/m L,壳聚糖(CS)质量浓度为2 mg/m L,CS溶液与三聚磷酸钠(TPP)溶液(1.0 mg/m L)的体积比为20∶3,所制备的GA-CS-NPs平均粒径(310.27±10.02)nm,包封率(51.42±0.43)%,载药量(6.87±0.47)%。质量评价结果表明,GA-CS-NPs外观圆整、均匀,在低温条件下,具有一定的稳定性。结论离子交联法制备GA-CS-NPs工艺简单、可靠,产品稳定性好。
Objective To study the optimal preparation process of glycyrrhetinic acid-chitosan nanoparticles（GA-CS-NPs） and to evaluate the quality.Methods The GA-CS-NPs were prepared by ionic cross-linking.The particle size,drug loading,and encapsulation efficiency were as evaluation indexes.The prescription and preparation process were optimized through single factor and orthogonal design.The quality of GA-CS-NPs was evaluated by morphology,particle size,drug loading,and encapsulation efficiency.Results The optimal prescription was as follows：concentration of GA and CS was 0.2 and 2 mg/mL,ratio of CS and TPP（1.0 mg/mL） solutions was 20：3.The mean diameter of GA-CS-NPs was（310.27±10.02） nm,the entrapment efficiency and drug-loading efficiency were（51.42±0.43）%and（6.87±0.47）%.The evaluation showed that the appearance of GA-CS-NPs was round and uniform.It had a certain stability under lower temperature.Conclusion The ionic cross-linking method used to prepare GA-CS-NPs is simple,rational,and stable.
出处
《中草药》
CAS
CSCD
北大核心
2015年第15期2232-2237,共6页
Chinese Traditional and Herbal Drugs
基金
甘肃省科技支撑计划-甘草次酸口服囊泡包裹的纳米载体构建及肝靶向特性研究(1204FKCA183)
关键词
甘草次酸
壳聚糖
纳米粒
离子交联法
正交设计
质量评价
glycyrrhetinic acid
chitosan
nanoparticles
ionic cross-linking
orthogonal design
quality assessment
