摘要
目的:观察皂荚提取物对肝癌大鼠TIMP3/MMPs蛋白的影响。方法:以皂荚提取物低、中、高剂量作用于肝癌模型大鼠,应用Western blot检测肝癌大鼠组织中TIMP3、MMP2及MMP9蛋白的表达水平。结果:皂荚提取物低、中、高剂量作用后的大鼠肝癌组织病理形态学得到明显改善,并且使组织中TIMP-3蛋白表达上调(均P<0.01),抑制MMP2(低剂量组P<0.05;中、高剂量组P<0.01)及MMP9(低剂量组P<0.05;中、高剂量组P<0.01)蛋白的表达。结论:皂荚提取物具有显著的抗肝癌作用,且该效应与影响TIMP3/MMPs蛋白有关,高剂量皂荚提取物效果最为显著。
Objective：To study the effect of Gleditsia sinesis extract to TIMP3/MMPs proteins in hepatoma model on rats. Methods：Gleditsia sinesis extract of low,medium and high doses was given to hepatoma model on rats,the protein expression of TIMP3,MMP2 and MMP9 were detected. Results：The pathology morphology of rat liver tissue was improved obviously after the effect of Gleditsia sinesis extract,and the expression level of TIMP3 was up-regulated（P 〈0.01）,MMP2 and MMP9 were inhibited（low：P 〈0.05,medium：P 〈0.01,high：P 〈0.01）. Conclusion：Gleditsia sinesis extract had anti-hepatocellular carcinoma effect,and the effect was related to TIMP3/MMPs,the high dose of Gleditsia sinesis extract was the most effective.
出处
《中西医结合肝病杂志》
CAS
2015年第4期224-226,I0001,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
湖北省自然科学基金项目(NO.2012FFC042)
