摘要
目的研究调节性T细胞新的表面标志物GARP mRNA及其蛋白在子宫内膜癌组织和正常子宫内膜组织中的表达及临床意义。方法应用免疫组化SP法及实时荧光定量PCR法检测84例子宫内膜癌组织和40例正常子宫内膜组织GARPmRNA及其蛋白的表达,分析GARP在子宫内膜癌组织中的表达与肿瘤的组织分化程度和FIGO分期的关系。结果子宫内膜癌组织GARP表达明显高于正常子宫内膜组织;GARP表达与子宫内膜癌FIGO分期有关,Ⅲ-Ⅳ期子宫内膜癌患者GARP表达高于Ⅰ-Ⅱ期患者（P〈0.01）。GARP表达与组织分化等级间存在相关关系（P〈0.01）,中-低分化组GARP＋表达明显高于高分化组（P〈0.01）。结论 GARP在子宫内膜癌患者癌灶中高表达,免疫组化的蛋白表达检测结果与实时荧光定量PCR检测结果一致,GARP表达与子宫内膜癌手术病理分期及组织分化有关,通过对GARP的检测可能会更客观准确地揭示Treg细胞与子宫内膜癌的发生、发展的关系。
Objective To research the expressions and clinical significance of GARP（ a surface marker of regulatory T cells） mRNA and its protein in endometrial carcinoma and normal endometrial tissue. Methods Immunohistochemical SP method and real- time fluorescence quantitative PCR were used to detect the expressions of GARP mRNA and protein in 84 cases of endometrial carcinoma and 40 cases of normal endometrial tissue. The relationship between GARP expression in endometrial carcinoma and histological differentiation degree,FIGO staging was analyzed. Results GARP expression in endometrial carcinoma was significantly higher than that in normal endometrial tissue. GARP expression was correlated with FIGO staging of endometrial carcinoma,GARP expression in endometrial carcinoma cases of stageⅢ- Ⅳ was statistically significantly higher than that in endometrial carcinoma cases of stage Ⅰ- Ⅱ（ P 〈0. 01）. GARP expression was correlated with histological differentiation degree（ P 〈0. 01）,GARP expression in poorly- differentiated endometrial carcinoma cases was statistically significantly higher than that in well- differentiated endometrial carcinoma cases（ P 〈0. 01）. Conclusion GARP expression in endometrial carcinoma cases is high. Immunohistochemical method and real- time fluorescence quantitative PCR show the same result in GARP protein expression. GARP expression is correlated with surgical- pathological staging and histological differentiation degree of endometrial carcinoma,GARP detection can more objectively and accurately reveal the relationship between regulatory T cells and occurrence and development of endometrial carcinoma.
出处
《中国妇幼保健》
CAS
2015年第26期4561-4564,共4页
Maternal and Child Health Care of China
基金
河北省科技支撑计划项目〔20122136〕
