摘要
通过下调人胃癌细胞BGC823中linc RNA HOTAIR基因的表达,该文探讨了linc RNA HOTAIR低表达对胃癌细胞迁移、侵袭及增殖能力的影响。该文构建针对人linc RNA HOTAIR基因的干扰质粒sh HOTAIR,稳定转染入胃癌细胞BGC823、筛选稳转株,q PCR检测linc RNA HOTAIR在胃癌细胞中表达水平。采用划痕试验、侵袭试验、MTT法分别检测转染胃癌细胞迁移、侵袭及增殖能力。结果表明,稳定转染干扰质粒sh HOTAIR后下调linc RNA HOTAIR表达的胃癌细胞株细胞迁移、侵袭及增殖能力较阴性对照组明显减弱。下调胃癌细胞中linc RNA HOTAIR的表达,可降低胃癌细胞的迁移力、侵袭性、抑制其增殖能力,提示linc RNA HOTAIR可作为分子靶点用于胃癌的分子靶向治疗。
This work was aim to study the effect of downregulating linc RNA HOTAIR expression on the migration and proliferation of BGC823 gastric cancer cells. The short hairpin RNA sh HOTAIR was transfected into BGC823 gastric cancer cells, and the stably transfected cells were isolated by G418 selection. The linc RNA HOTAIR gene expression was detected by q PCR. The abilities of migration, invasion and proliferation of sh HOTAIR transfected gastric cancer cells were investigated in the ways of wound healing and transwell invasion and MTT assays. The results showed that the abilities of migration, invasion and proliferation of the gastric cancer cells of low linc RNA HOTAIR expression were significantly decreased compared with the control group. Targeting linc RNA HOTAIR expression in gastric cancer cells with RNA interference could significantly reduce and inhibit the migration and proliferation abilities of gastric cancer cells. These data suggested that linc RNA HOTAIR could be one potential therapeutic target for human gastric caner treatment.
出处
《中国细胞生物学学报》
CAS
CSCD
2015年第8期1081-1086,共6页
Chinese Journal of Cell Biology
基金
蚌埠医学院科技发展基金重点项目(批准号:Bykf13A08)资助的课题~~
