摘要
以采自云南昭通的66株野生紫丁香蘑（Lepista nuda,L.nuda）为研究对象,分析了其ITS序列及其单核苷酸多态性（SNP）。结果表明：经结合形态和分子水平的鉴定,确定所采的野生蘑菇为紫丁香蘑;66个紫丁香蘑的ITS序列长度变化范围为666~671bp,ITS1为241~244bp,5.8S为155bp,ITS2为267~275bp,G＋C含量的变化范围为41.5%~42.4%,所有样本的平均遗传距离为0.003 0;通过序列比对,共筛选出7个SNP位点,单突变位点3个,简约信息位点4个;转换与颠换的比值R为0.672;该群体的核酸多态性指数Pi为0.002 91,每位点核苷酸多态性指数Theta（per site from Eta）为0.002 36。采用邻接法（neighbour joining,NJ）构建的系统发育树分支支持率低,表明群体内各菌株遗传距离小,该群体的遗传多样性丰度较低。综上说明云南昭通野生紫丁香蘑群体的遗传多样性水平很低,需要加强保护。
Lepista nuda from Zhaotong,Yunnan Province was used as the research object,its ITS sequence and single nucleotide polymorphism（SNP）were analyzed.The results showed that：combined with morphological and molecular identification,the collected wild mushrooms were confirmed as Lepista nuda;66strains L.nuda＇s ITS sequence length was 666-671 bp,ITS1：241-244 bp,5.8S：155bp,ITS2：267-275bp;G＋C content was 41.5%-42.4%;the average genetic distance of all samples was 0.003 0.By sequence alignment,seven SNPs loci were screened out：three singleton variable sites,four parsimony informative sites;transition and transversion ratio was 0.672.The nucleic acid polymorphisms index Pi of the population was 0.002 91.Each site nucleotide polymorphisms index Eta was 0.002 36.Neighbor（neighbour-joining,NJ）phylogenetic tree was constructed.Bootstrap support rate was low,which indicated that genetic distance of each strain in the population was very close and their genetic diversity was very low.In summary,the genetic diversity of the wild Lepista nuda population from Zhaotong was very low so that its protection should be strengthened.
出处
《北方园艺》
CAS
北大核心
2015年第17期132-136,共5页
Northern Horticulture
基金
云南省优势特色重点学科生物学一级学科建设资助项目(50097505)
云南省应用基础研究计划资助项目(2011FZ141)
西南地区生物多样性保育国家林业局重点实验室开放基金资助项目(KL200707)
云南省高校特色专业建设点资助项目(50116001)
关键词
紫丁香蘑
ITS
SNP
遗传多样性
Lepista nuda
internal transcribed spacers（ITS）
single nucleotide polymorphism（SNPs）
genetic diversity
