摘要
巨大口蘑是一种耐储存的珍稀食用菌,为了研究巨大口蘑抗褐变耐储存的机理,以巨大口蘑子实体为实验材料,以无处理和清水处理为对照,研究硫酸亚铁(5 g/L)处理对巨大口蘑储存过程中的褐变度、PPO、TYE、PAL、总酚、SOD、CAT、POD、MDA的影响。结果表明:在12℃储存条件下,Fe SO4处理可以明显加快巨大口蘑子实体褐变速度,明显促进了PPO、TYE、SOD、POD的升高,抑制了PAL、总酚的上升趋势,导致巨大口蘑细胞中细胞膜被破坏,通透性增强,导致子实体褐变速度加快;无处理对照样品和清水处理中PPO、TYE、PAL、总酚含量较低,SOD、POD维持较高水平,MDA含量较低,细胞膜完整,从而降低了褐变速度,延长了储存期。
Tricholoma giganteum is one of the most anti-browning mushrooms. In order to explore the mechanism of Tricholoma giganteum anti-browning After being treated with ferrous sulfate （FeSO4） （5 g/ L） or water, these factors were investigated, such as browning degree （BD）, activity of polyphenol oxidase （PPO）, tyrosinase （TYE） and phenylalnine ammonialyase （PAL）, content of phenolic, activity of superoxide dismutase （SOD）, catalase （CAT）, and peroxidase （POD）, malondialdehyde （MDA）. Result revealed that Fe（H） ions caused an increase in PPO, TYE, MDA, membrane were damaged by free radical, BD was higher than no treatment and water treatment, SOD, POD were irritated in the storage of former and PAL were inhibited. There was little PPO, TYE in no treatment, with highly level of SOD, the membrane of no treatment fruit bodies were protected well, PPO and TYE cannot contact with phenolic. These results indicated that excess Fe（Ⅱ） ions produced damange in T. giganteum cell membrane and enzymatic browning was speed up. While high level SOD, POD, AsA protected celt membrane in no treatment fruit and BD was minimized.
出处
《食品科技》
CAS
北大核心
2015年第8期350-354,共5页
Food Science and Technology
基金
国家自然科学基金项目(31272218)
国家星火计划项目(2012GA780042
2013GA780040)
广东省农业科技推广专项(201201138)
广州市花都区产学研结合专项(HD13CXY-010)
广东省科技计划项目
关键词
巨大口蘑
抗褐变机制
离子处理
酶活力
Tricholoma giganteum
anti-browning mechanism
ions stress
enzyme activity
