摘要
目的探讨Th17及相关细胞因子在血吸虫病肝纤维化中的表达及意义。方法采用流式细胞技术(FCM)检测血吸虫病肝纤维化患者外周血Th17细胞占CD4+T细胞的比例;用酶联免疫吸附测定法(ELISA)检测患者血清细胞因子白细胞介素17(IL-17)和转化生长因子TGF-β1的表达水平,以健康者作对照组比较观察。结果血吸虫病肝纤维化患者外周血Th17细胞比率为(2.86±1.23)%,明显高于健康对照组(1.07±0.93)%,差异有统计学意义(P<0.05)。血吸虫病肝纤维化患者血清IL-17浓度为21.86±3.47ng/L,显著高于对照组11.37±2.59ng/L,差异有统计学意义(P<0.05);血清TGF-β1浓度为296.46±47.35ng/L,显著高于对照组185.53±35.23ng/L,差异有统计学意义(P<0.05)。血吸虫病肝纤维化患者血清IL-17含量与TGF-β1水平呈正相关(r=0.303,P<0.05)。结论血吸虫病肝纤维化患者Th17及IL-17、TGF-β1呈现高表达,提示Th17及相关细胞因子可能参与了血吸虫病肝纤维化的免疫病理损害及肝虫卵肉芽肿形成。
Objective To investigate the variation of the proportions of peripheral blood Th17 cells as well as the serum level of Th17-related cytokines in the patients infected with Schistosoma japonicum. Methods The proportions of peripheral blood Th17 cells in the patients infected with Schistosoma japonicum and healthy subjects were determined by flow cytometry（ FCM）. ELISA was used to detect IL-17 and TGF-β1 in serum. Results The proportion of CD4＋T cells in patients infected with Schistosoma japonicum was 2. 86 ± 1. 23 %,significantly higher than that in the healthy controls（ 1. 07 ± 0. 93 %）,（ P〈0. 05）. The levels of IL-17 and TGF-β1 in serum of the patient groups were higher,the difference was significant（ P〈0. 05）,（ r = 0. 303,P〈0. 05）. IL-17 and TGF-β1 levels in the serum of the patients were positively correlated. Conclusion Th17 and the Th17-related cytokines IL-17 and TGF-β1 increased after the formation of Schistosomiasis liver fibrosis,which suggested that Th17 and the Th17-related cytokines might be involved in the pathogenesis of immune pathological damage of schistosomiasis liver fibrosis and hepatic schistosoma egg granuloma formation.
出处
《公共卫生与预防医学》
2015年第4期29-31,共3页
Journal of Public Health and Preventive Medicine
基金
武汉市卫生和计划生育委员会临床医学科研项目(WX13C44)
交通运输部长江航道局重点科技项目(201330011)
