摘要
以秸秆为基料对滑菇进行富锌栽培,通过对富锌栽培的滑菇的纤维素酶及漆酶活力的测定,结合生物转化率,研究滑菇在不同秸秆和木屑比例的基料上的纤维素酶及漆酶的变化规律,探索用秸秆代替木屑进行滑菇栽培的可行性,为秸秆的回收利用提供新的途径。实验结果表明,最适宜滑菇生长的培养基料配比为秸秆与木屑质量比为1∶1,生物转化率达41.23%,比纯木屑栽培提高25.14%,富锌栽培使生物转化率提高14.25%,在菌丝生长时期纤维素酶活均高于纯木屑基料纤维素酶活,其中滤纸酶活提高28.57%,羧甲基纤维素酶活提高84.68%,半纤维素酶活提高17.70%,β-葡萄糖苷酶活力提高60.00%。
Using straw as base material to culture Zinc rich Pholiota nameko,the activity of its cellulase and laccase,the biological conversion rate,the change rules of cellulase and laccase based on different proportion of straw and sawdust were studied. The feasibility of using wood chips instead of straw mushroom cultivation slip was explored,which provided a new way for recycling straw. The experimental results showed that the optimum ratio of straw and sawdust was 1∶ 1 in Pholiota nameko culture medium. The biological conversion rate was 41. 23%,increased by 25.14% compared with pure sawdust cultivation. Zinc rich cultivation biological conversion rate was increased by 14.25%. The activity of cellulase was higher than that of cellulase from pure wood base material culture in the mycelial growth period,such as the activity of filter paper enzymeincreased by 28. 57%,the activity of carboxymethyl cellulase increased by 84. 68%,the activity of hemicellulase increased by 17. 70%,and the activity of β-glucosidase increased by 60%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第4期121-124,共4页
Food and Fermentation Industries
基金
大连民族学院大学生创新创业训练计划项目(X201403053)
大连民族学院学生太阳鸟项目(tyn2014076)
2015年大连民族学院自主科研基金民族地区与地方经济计划项目
关键词
滑菇
秸秆
富锌
纤维素酶
漆酶
Pholiota nameko
straw
Zinc-riching
cellulase
laccase
