摘要
目的观察电痉挛刺激(ECS)对Sprague-Dawley大鼠黑质酪氨酸羟化酶(TH)和中缝核色氨酸羟化酶(TPH)表达的变化,探讨ECS对黑质多巴胺和中缝核5-羟色胺神经元活性的影响。方法将大鼠随机分为对照组、伪ECS组、单次ECS组和重复ECS组。对照组大鼠未给予任何处理,伪ECS组大鼠给予不通电ECS,单次ECT组大鼠给予1次ECS,重复ECS组大鼠给予每日1次ECS,连续14 d。运用免疫荧光技术检测黑质TH和中缝核TPH的阳性细胞数和平均吸光度值。结果免疫荧光技术检测结果显示,对照组、伪ECS组、单次ECS组大鼠黑质TH和中缝核TPH阳性表达神经元个数和神经元纤维光强度比较差异均无统计学意义(P>0.05),而重复ECS组大鼠黑质TH和中缝核TPH阳性表达神经元个数较对照组、伪ECS组和单次ECS组增多(P<0.05),神经纤维光强度较对照组、伪ECS组、单次ECS组增加(P<0.05)。结论重复ECS可促进黑质TH和中缝核TPH的表达,表明重复ECS上调了多巴胺和5-羟色胺神经元的活性。
Objective To observe the effect of electroconvulsive seizure （ECS） on the activity of substantia nigra do- paminergic（DA）,and rapheal nuclei serotonin （5-HT） neurons by investigating the expression levels of tyrosine hydroxylase （TH） in the substantia nigra and tryptophan hydroxylase（TPH） in the rapheal nuclei of Sprague-Dawley rats after ECS. Meth- otis Rats were randomly divided into control group, sham ECS group, single ECS group and repeated ECS group. The rats in control group received no handling;the rats in sham ECS group were identically handled and given electric stimulation without electricity;the rats in single ECS group were given one shock;the rats in repeated ECS group were total given 14 shocks,once daily for 14 consecutive days. The mean density and positive neuron number of TH in the substantia nigra and TPH in the ra- pheal nuclei were detected by using immunofluorescence. Results Immunofluorescence staining result showed that there was no significant difference of TH and TPH-positive neuron number and TH and TPH-mean density value among control group,sham ECS group and single ECS group（P 〉0.05）. The positive neuron number and mean density value of TH in the substantia nigra and TPH in the rapheal nuclei in repeated ECS group were higher than those in control group, sham ECS group and single ECS group （P 〈 0.05）. Conclusion Repeated ECS can promote the expression of TH in the substantia nigra and TPH in the rapheal nuclei ,which shows repeated ECS up-regalation the activity of DA and 5-HT neurons.
出处
《新乡医学院学报》
CAS
2015年第8期728-731,共4页
Journal of Xinxiang Medical University
关键词
电痉挛刺激
多巴胺
5-羟色胺
酪氨酸羟化酶
色氨酸羟化酶
electroconvulsive stimulus
dopaminergic
serotonin
tyrosine hydroxylase
tryptophan hydroxylase
