摘要
目的探讨多重荧光PCR用于检测男性不育Y染色体微缺失的效果及临床意义。方法本实验采用多重荧光PCR对120例男性不育患者进行Y染色体微缺失检测。结果在120例男性不育患者中共检测出有缺失者7例(AZFc区缺失5例,AZFb+c区缺失2例),总缺失率为5.8%。有Y染色体缺失的患者均表现出无精或少精症状,其中48例无精症患者有5例缺失,缺失率为10.4%;24例少精症患者中有2例缺失,缺失率为8.3%。结论多重荧光PCR方法操作方便,结果准确,灵敏度高,重复性好,在Y染色体微缺失检测上有重要应用价值。
Objective：To investigate effect and clinical significance of multiplex fluorescent PCR in detection of Y chromosomal microdeletions in infertile men. Methods：In this study,120 infertile men were detected of Y chromosome microdeletion by multiplex fluorescent PCR. Results：There were 7 cases of 120 patients have Y chromosomal microdeletions（5cases was detected in AZFc region,2 cases was detected in AZFb＋c region）in the patients,the total rate of microdeletions was 5.8%. All microdeletions were occurred in patients with azoospermia or oligospermia,and the deletion rates were 10.4%（5/48）in azoospermia and 8.3%（2/24）in oligospermia. Conclusion：Multiplex fluorescent PCR method is convenient,accurate,sensitive and reproducible,and has important application value in the detection of Y chromosome microdeletions.
出处
《中国优生与遗传杂志》
2015年第8期5-7,20,共4页
Chinese Journal of Birth Health & Heredity
基金
重庆市自然科学基金(csts2012jj A0913)
关键词
Y染色体微缺失
多重荧光PCR
应用
Y chromosome microdeletions
Multiplex fluorescent PCR
Application
