摘要
【目的】构建禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)菌株APEC-O1空泡形成毒素(Vacuolating autotransporter toxin,Vat)基因缺失株,研究该基因对APEC-O1生物学特性及致病性的影响。【方法】采用Red同源重组的方法构建vat缺失株,并利用低拷贝质粒p STV28构建互补株,然后比较野生株、vat缺失株与互补株在生长特性、运动性、凝集沉淀能力、生物被膜、致病性等生物学特性的差异。【结果】vat基因缺失不影响APEC-O1的生长速度及抗环境压力能力,但缺失vat导致APEC-O1运动能力增强,而使生物被膜形成能力、凝集沉降能力、致病力、体内存活能力均显著性减弱。【结论】Vat缺失影响APEC-O1运动能力、凝集沉淀能力、生物被膜形成能力及致病力,为全面了解Vat的致病作用提供参考。
[ Objective ] To determined the role of vacuolating autotransporter toxin （vat） gene in avian pathogenic Escherichia coli （APEC） , we detected the biological characteristics and pathogenicity of vat gene mutant strain of APEC- O1. [ Methods] We constructed the vat mutant and complementary strain of APEC-O1 by the Red recombination system and plasmid pSTV28. Then we compared the growth curve, motility, agglutination, biofilm formation and pathogenicity of mutant strain, wild-type strain and complementary strain. [ Results] The vat mutant did not affect the growth and resistance to environment stress of APEC. However, inactivation of APEC-O1 vat gene resulted in enhanced motility, diminished agglutination, decreased biofilm formation and attenuated virulence in ducks. [ Conclusion ] These data indicated that Vat affect the motility, agglutination, biofilm formation and virulence of APEC-O1, which help us to understand the role of the Vat in the APEC pathogenicity.
出处
《微生物学报》
CAS
CSCD
北大核心
2015年第9期1208-1214,共7页
Acta Microbiologica Sinica
基金
公益性农业(科研)专项(201303044)
国家自然科学基金(81201266,31370045)
中央级科研院所基本科研业务费项目(2015JB08)
上海市科技兴农重点攻关项目[沪农科攻字(2015)第1-9号]~~
关键词
禽致病性大肠杆菌
空泡转运毒素
基因缺失
生物学特性
致病性
avian pathogenic Escherichia coli, vacuolating autotransporter toxin, gene mutant, biological characteristics, pathogenicity
